IL-4 driven transcription factor FoxQ1 is expressed by monocytes in atopic dermatitis and stimulates monocyte migration

Ilja Ovsiy, Vladimir Riabov, Ioannis Manousaridis, Julia Michel, Kondaiah Moganti, Shuiping Yin, Tengfei Liu, Carsten Sticht, Elisabeth Kremmer, Martin C. Harmsen, Sergij Goerdt, Alexei Gratchev, Julia Kzhyshkowska*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

4 Citations (Scopus)
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Abstract

Monocytes are actively recruited at sites of chronic inflammation. However, molecular factors involved in this process are not fully elucidated. Here, we show that cytokine IL-4 which is implicated in the development of chronic inflammatory disease atopic dermatitis (AD) induces expression of transcription factor FoxQ1 in human monocytes and macrophages. FoxQ1 mRNA levels were elevated in monocytes of AD patients compared to healthy donors. Overexpression of FoxQ1 in RAW 264.7 monocytic cells facilitated their migration towards MCP-1 and was associated with decreased expression of migration-regulating genes (claudin 11 and plexin C1). Furthermore, FoxQ1 overexpression in RAW cells accelerated TNF alpha secretion after LPS challenge. Overall, our results indicate that FoxQ1 stimulates monocyte motility, increases pro-inflammatory potential, and directs monocyte migration towards MCP-1 that is crucial for monocyte influx into inflammatory sites. This mechanism could contribute to the pathogenesis of chronic inflammatory disorders such as AD.

Original languageEnglish
Article number16847
Number of pages9
JournalScientific Reports
Volume7
Issue number1
DOIs
Publication statusPublished - 4-Dec-2017

Keywords

  • ENDOTHELIAL-CELLS
  • RECEPTOR-TYPE
  • INFLAMMATION
  • RECRUITMENT
  • MACROPHAGES
  • CANCER
  • INTERLEUKIN-4
  • HETEROGENEITY
  • EOSINOPHILS
  • METASTASIS

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