Abstract
The application of immunoaffinity chromatography for the purification of Taxus plant and cell extracts prior to the HPLC analysis is described. Polyclonal antibodies raised against 10-deacetylbaccatin III (10-DAB III), paclitaxel's main precursor in plant, were characterised by enzymed-linked immunosorbent assay. Immunoglobulins from selected antisera were immobilised on CNBr-activated Sepharose 4B. The immunoaffinity column was used for the purification of plant and plant cell culture extracts prior to their analysis by HPLC. Immunoaffinity chromatography enabled the selective concentration of taxoids and enhanced sample clean-up. (C) 2002 Elsevier Science B.V. All rights reserved.
| Original language | English |
|---|---|
| Article number | PII S0021-9673(01)01456-X |
| Pages (from-to) | 177-185 |
| Number of pages | 9 |
| Journal | Journal of Chromatography A |
| Volume | 948 |
| Issue number | 1-2 |
| Publication status | Published - 1-Mar-2002 |
| Event | 25th International Symposium on High Performance Liquid Phase Separations and Related Techniques - , Netherlands Duration: 17-Jun-2001 → 22-Jun-2001 |
Keywords
- Taxus spp.
- immunoaffinity chromatography
- sample handling
- plant materials
- taxol
- paclitaxel
- deacetylbaccatin III
- taxanes
- DIPHOSPHATE SYNTHASE ACTIVITY
- AFFINITY-CHROMATOGRAPHY
- PHENYLUREA HERBICIDES
- TRIAZINE HERBICIDES
- RESIDUE ANALYSIS
- TAXOL
- CLEANUP
- CULTURES
- BACCATA
- CHLORTOLURON