Immunochemical localization of ribulose-1,5-bisphosphate carboxylase in the symbiont-containing gills of Solemya velum (Bivalvia: Mollusca)

Colleen M. Cavanaugh, Marilyn S. Abbott, Marten Veenhuis

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Abstract

The distribution of the Calvin cycle enzyme ribulose-1,5-bisphosphate carboxylase (RbuP2Case; EC 4.1.1.39) was examined by using two immunological methods in tissues of Solemya velum, an Atlantic coast bivalve containing putative chemoautotrophic symbionts. Antibodies elicited by the purified large subunit of RbuP2Case from tobacco (Nicotana tabacum) cross-reacted on immunoblots with a protein of similar molecular mass occurring in extracts of the symbiont-containing gill tissue of S. velum. No cross-reactivity was detected in symbiont-free tissue extracts. The antiserum also cross-reacted in immunoblots with proteins of Thiobacillus neapolitanus, a free-living sulfur-oxidizing chemoautotroph whose RbuP2Case has been well characterized. In protein A-gold immunoelectron microscopy studies, this antiserum consistently labeled the symbionts but not surrounding host gill tissue, indicating that the symbionts are responsible for the RbuP2Case activity.
Original languageEnglish
Number of pages4
JournalProceedings of the National Academy of Sciences
Volume85
Issue number20
DOIs
Publication statusPublished - 1988

Keywords

  • symbiosis
  • deep-sea hydrothermal vents
  • sulfur-oxidizing bacteria
  • chemoautotroph

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