Immunohistopathological appearance of three different types of injury in human skin

N van der Laan, LFMH de Leij, HJ ten Duis*

*Corresponding author for this work

    Research output: Contribution to journalArticleAcademicpeer-review

    12 Citations (Scopus)

    Abstract

    Objective and design: To determine whether underlying mechanisms of inflammation, like cellular infiltrates, expression of adhesion molecule and cytokine patterns are similar under different conditions of injury. Skin biopsies were taken of three different groups of patients in which local inflammation of the skin might occur.

    Material, patients: Skin activation was studied as a result of incision during surgery under aseptic conditions, as a result of a local bacterial infection and as a result of blunt trauma, resulting in a femoral fracture, without disruption of the epithelial barrier.

    Methods: Skin biopsies were snap frozen for immunohistochemical analysis.

    Results: Incision of the skin resulted in a granulocyte infiltrate, paralleled by E-selectin expression. As a result of infection granulocytes were observed and monocyte/macrophage and T cell numbers were increased. Furthermore, E-selectin, VCAM-1 and ICAM-1 expression increased and cytokine expression markedly changed compared to normal skin. Skin taken at the site of the femoral fracture showed no signs of inflammation.

    Conclusion: Different stimuli lead to different local inflammatory responses in human skin.

    Original languageEnglish
    Pages (from-to)350-356
    Number of pages7
    JournalInflammation research
    Volume50
    Issue number7
    Publication statusPublished - Jul-2001

    Keywords

    • inflammation
    • skin
    • adhesion molecules
    • cytokines
    • injury
    • ENDOTHELIAL ADHESION MOLECULES
    • CELL-ADHESION
    • LEUKOCYTE RECRUITMENT
    • UP-REGULATION
    • CHEMOKINES
    • MECHANISMS
    • EXPRESSION
    • CYTOKINES
    • INFLAMMATION
    • SELECTINS

    Cite this