TY - JOUR
T1 - Immunological Detection of G-Protein α-Subunits in Dictyostelium discoideum
AU - Snaar-Jagalska, B. Ewa
AU - Kesbeke, Fanja
AU - Pupillo, Maureen
AU - Haastert, Peter J.M. van
N1 - Relation: http://www.rug.nl/gbb/
date_submitted:2007
Rights: University of Groningen, Groningen Biomolecular Sciences and Biotechnology Institute
PY - 1988
Y1 - 1988
N2 - Putative G-protein α-subunits in Dictyostelium discoideum were detected on western blots using the antiserum A-569, raised against a peptide whose sequence is found in α-subunits of all known GTP-binding signal transducing proteins. Two bands with a MW of 40 kDa and 52 kDa were specifically recognized by the common peptide antiserum; the staining of both bands was strongly reduced when the antiserum was preincubated with the peptide that was used for antibody production. D. discoideum mutant HC213 (fgd A) lacks staining of the 40 kDa band, while the 52 kDa band is still present. This mutant is severely defective in cAMP receptor-G-protein interaction. We concluded that the primitive eukaryote D. discoideum contains proteins which show functional and physical similarity with the α-subunits of vertebrate G-proteins.
AB - Putative G-protein α-subunits in Dictyostelium discoideum were detected on western blots using the antiserum A-569, raised against a peptide whose sequence is found in α-subunits of all known GTP-binding signal transducing proteins. Two bands with a MW of 40 kDa and 52 kDa were specifically recognized by the common peptide antiserum; the staining of both bands was strongly reduced when the antiserum was preincubated with the peptide that was used for antibody production. D. discoideum mutant HC213 (fgd A) lacks staining of the 40 kDa band, while the 52 kDa band is still present. This mutant is severely defective in cAMP receptor-G-protein interaction. We concluded that the primitive eukaryote D. discoideum contains proteins which show functional and physical similarity with the α-subunits of vertebrate G-proteins.
U2 - 10.1016/S0006-291X(88)80908-2
DO - 10.1016/S0006-291X(88)80908-2
M3 - Article
VL - 156
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 2
ER -