Implementation of the agmatine-controlled expression system for inducible gene expression in Lactococcus lactis

Daniel M Linares, Patricia Alvarez-Sieiro, Beatriz del Rio, Victor Ladero, Begoña Redruello, Ma Cruz Martin, Maria Fernandez, Miguel A Alvarez

Research output: Contribution to journalArticleAcademicpeer-review

10 Citations (Scopus)
41 Downloads (Pure)

Abstract

Background: Lactococcus lactis has been safely consumed in fermented foods for millennia. This Gram-positive bacterium has now become of industrial importance as an expression host for the overproduction of lipopolysaccharide-free recombinant proteins used as food ingredients, therapeutic proteins and biotechnological enzymes.

Results: This paper reports an agmatine-controlled expression (ACE) system for L. lactis, comprising the lactococcal agmatine-sensor/transcriptional activator AguR and its target promoter P-aguB. The usefulness and efficiency of this system was checked via the reporter gene gfp and by producing PEP (Myxococcus xanthus prolyl-endopeptidase), an enzyme of biomedical interest able to degrade the immunotoxic peptides produced during the gastrointestinal breakdown of gluten.

Conclusion: The ACE system developed in this work was suitable for the efficient expression of the functional recombinant proteins GFP and PEP. The expression system was tightly regulated by the agmatine concentration and allowed high protein production without leakiness.

Original languageEnglish
Article number208
Number of pages12
JournalMicrobial Cell Factories
Volume14
DOIs
Publication statusPublished - 30-Dec-2015
Externally publishedYes

Keywords

  • Lactococcus lactis
  • Expression vector
  • Agmatine induction
  • Myxococcus xanthus
  • Prolyl-endopeptidase
  • RECOMBINANT PROTEIN-PRODUCTION
  • MEMBRANE-PROTEINS
  • ACID BACTERIA
  • IMMUNE-RESPONSES
  • VACCINE DELIVERY
  • NISIN
  • BIOSYNTHESIS
  • MUCOSAL
  • OVERPRODUCTION
  • TRANSCRIPTION

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