Improved means and methods for expressing recombinant proteins

Berend Poolman; Daniel Martinez Linares; Nadia Gul

Improved means and methods for expressing recombinant proteins

Berend Poolman (Inventor), Daniel Martinez Linares (Inventor), Nadia Gul (Inventor)

Enzymology

Research output: Patent

Abstract

The invention relates to the field of genetic engineering and the production of recombinant proteins in microbial host cells. Provided is a method for enhanced expression of a recombinant protein of interest in a microbial host cell, comprising providing a microbial host cell wherein the function of H-NS (histone-like nucleoid structuring protein) or a homolog thereof is impaired; transforming the host cell with an expression vector comprising a nucleotide sequence encoding the recombinant protein; and culturing the transformed host cell in a culture media suitable for the expression of the recombinant protein.

Original language	English
Patent number	EP2796556
Priority date	25/04/2013
Publication status	Published - 29-Oct-2014

Access to Document

https://nl.espacenet.com/publicationDetails/originalDocument?CC=EP&NR=2796556A1&KC=A1&FT=D&ND=3&date=20141029&DB=&locale=nl_NL

Handle.net

Persistent link

Cite this

@misc{3bbc7d2517e8487d9646579fd11d8caf,

title = "Improved means and methods for expressing recombinant proteins",

abstract = "The invention relates to the field of genetic engineering and the production of recombinant proteins in microbial host cells. Provided is a method for enhanced expression of a recombinant protein of interest in a microbial host cell, comprising providing a microbial host cell wherein the function of H-NS (histone-like nucleoid structuring protein) or a homolog thereof is impaired; transforming the host cell with an expression vector comprising a nucleotide sequence encoding the recombinant protein; and culturing the transformed host cell in a culture media suitable for the expression of the recombinant protein.",

author = "Berend Poolman and {Martinez Linares}, Daniel and Nadia Gul",

year = "2014",

month = oct,

day = "29",

language = "English",

type = "Patent",

note = "EP2796556",

}

TY - PAT

T1 - Improved means and methods for expressing recombinant proteins

AU - Poolman, Berend

AU - Martinez Linares, Daniel

AU - Gul, Nadia

PY - 2014/10/29

Y1 - 2014/10/29

N2 - The invention relates to the field of genetic engineering and the production of recombinant proteins in microbial host cells. Provided is a method for enhanced expression of a recombinant protein of interest in a microbial host cell, comprising providing a microbial host cell wherein the function of H-NS (histone-like nucleoid structuring protein) or a homolog thereof is impaired; transforming the host cell with an expression vector comprising a nucleotide sequence encoding the recombinant protein; and culturing the transformed host cell in a culture media suitable for the expression of the recombinant protein.

AB - The invention relates to the field of genetic engineering and the production of recombinant proteins in microbial host cells. Provided is a method for enhanced expression of a recombinant protein of interest in a microbial host cell, comprising providing a microbial host cell wherein the function of H-NS (histone-like nucleoid structuring protein) or a homolog thereof is impaired; transforming the host cell with an expression vector comprising a nucleotide sequence encoding the recombinant protein; and culturing the transformed host cell in a culture media suitable for the expression of the recombinant protein.

M3 - Patent

M1 - EP2796556

ER -

Improved means and methods for expressing recombinant proteins

Abstract

Access to Document

Handle.net

Fingerprint

Cite this