Improved methodology for the affinity isolation of human protein complexes expressed at near endogenous levels

Michal Domanski, Kelly Molloy, Hua Jiang, Brian T Chait, Michael P Rout, Torben Heick Jensen, John LaCava*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

An efficient and reliable procedure for the capture of affinity-tagged proteins and associated complexes from human cell lines is reported. Through multiple optimizations, high yield and low background affinity-purifications are achieved from modest quantities of human cells expressing endogenous-level tagged proteins. Isolations of triple-FLAG and GFP-tagged fusion proteins involved in RNA metabolism are presented.

Original languageEnglish
Pages (from-to)1-6
Number of pages6
JournalBiotechniques
Volume0
Issue number0
DOIs
Publication statusPublished - May-2012
Externally publishedYes

Cite this