Improvement of in vivo transfer of plasmid DNA in muscle: Comparison of electroporation versus ultrasound

Yoka H. Kusumanto, Nanno H. Mulder, Wendy A. Dam, Mario H. Losen, Coby Meijer, Geke A. P. Hospers*

*Corresponding author for this work

    Research output: Contribution to journalArticleAcademicpeer-review

    25 Citations (Scopus)

    Abstract

    Plasmid-based gene delivery to muscle is a treatment strategy for many diseases with potential advantages above viral-based gene delivery methods, however, with a relative low transfection efficiency. We compared two physical methods-electroporation and ultrasound-that facilitate DNA uptake into cells. Mice (C57BI/6) were injected intramuscular using plasmid DNA encoding an intracellular protein (p53) followed by electroporation or ultrasound. Then 48 hr after the injections the mice were sacrificed. The parameter for transfection efficiency was the area of muscle expressing the transgene. The p53 expression plasmid showed a 36-fold increase (p = 0.015) in transfection efficiency with electroporation compared to ultrasound. Compared with ultrasound, electroporation significantly improves transfection efficiency of naked plasmid DNA transfer into skeletal muscle.

    Original languageEnglish
    Pages (from-to)273-277
    Number of pages5
    JournalDrug Delivery
    Volume14
    Issue number5
    DOIs
    Publication statusPublished - Jul-2007

    Keywords

    • electroporation
    • muscle
    • mouse model
    • plasmid DNA
    • ultrasound
    • MEDIATED GENE-TRANSFER
    • SKELETAL-MUSCLE
    • MOUSE MUSCLE
    • LONG-TERM
    • DELIVERY
    • OPTIMIZATION
    • EXPRESSION
    • CELLS
    • MODEL
    • MICE

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