Improvement of oxygen supply by an artificial carrier in combination with normobaric oxygenation decreases the volume of tissue hypoxia and tissue damage from transient focal cerebral ischemia

David J. Seiffge; Natalia E. Lapina; Charalambos Tsagogiorgas; Bastian Theisinger; Robert H. Henning; Lothar Schilling

doi:10.1016/j.expneuro1.2012.06.007

Improvement of oxygen supply by an artificial carrier in combination with normobaric oxygenation decreases the volume of tissue hypoxia and tissue damage from transient focal cerebral ischemia

David J. Seiffge, Natalia E. Lapina, Charalambos Tsagogiorgas, Bastian Theisinger, Robert H. Henning, Lothar Schilling^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

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Abstract

Tissue hypoxia may play an important role in the development of ischemic brain damage. In the present study we investigated in a rat model of transient focal brain ischemia the neuroprotective effects of increasing the blood oxygen transport capacity by applying a semifluorinated alkane (SFA)-containing emulsion together with normobaric hyperoxygenation (NBO). The spread of tissue hypoxia was studied using pimonidazole given prior to filament-induced middle cerebral artery occlusion (MCAO, 2 h). Treatment consisted of intravenous injection of saline or the SFA-containing emulsion (0.5 or 1.0 ml/100 g body weight; [SFA(0.5) or SFA(1.0)]) either upon establishing MCAO (early treatment) or after filament removal (delayed treatment). After injection NBO was administered for 8 h (early treatment) or 6 h (delayed treatment). Experiments were terminated 8 or 24 h after MCAO. In serial brain sections tissue hypoxia and irreversible cell damage were quantitatively determined. Furthermore, we studied hypoxia-related gene expression (VEGF, flt-1). Early treatment significantly (p

Original language	English
Pages (from-to)	18-25
Number of pages	8
Journal	Experimental Neurology
Volume	237
Issue number	1
DOIs	https://doi.org/10.1016/j.expneuro1.2012.06.007
Publication status	Published - Sept-2012

Keywords

Artificial oxygen carrier
Focal brain ischemia
Normobaric hyperoxygenation
Rat
Tissue hypoxia
ARTERY OCCLUSION
RAT-BRAIN
PERFLUOROCARBON EMULSIONS
HYPERBARIC OXYGENATION
REPERFUSION
EXPRESSION
MODEL
PERFUSION
THERAPY
FLUOSOL

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Seiffge, D. J., Lapina, N. E., Tsagogiorgas, C., Theisinger, B., Henning, R. H., & Schilling, L. (2012). Improvement of oxygen supply by an artificial carrier in combination with normobaric oxygenation decreases the volume of tissue hypoxia and tissue damage from transient focal cerebral ischemia. Experimental Neurology, 237(1), 18-25. https://doi.org/10.1016/j.expneuro1.2012.06.007

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title = "Improvement of oxygen supply by an artificial carrier in combination with normobaric oxygenation decreases the volume of tissue hypoxia and tissue damage from transient focal cerebral ischemia",

abstract = "Tissue hypoxia may play an important role in the development of ischemic brain damage. In the present study we investigated in a rat model of transient focal brain ischemia the neuroprotective effects of increasing the blood oxygen transport capacity by applying a semifluorinated alkane (SFA)-containing emulsion together with normobaric hyperoxygenation (NBO). The spread of tissue hypoxia was studied using pimonidazole given prior to filament-induced middle cerebral artery occlusion (MCAO, 2 h). Treatment consisted of intravenous injection of saline or the SFA-containing emulsion (0.5 or 1.0 ml/100 g body weight; [SFA(0.5) or SFA(1.0)]) either upon establishing MCAO (early treatment) or after filament removal (delayed treatment). After injection NBO was administered for 8 h (early treatment) or 6 h (delayed treatment). Experiments were terminated 8 or 24 h after MCAO. In serial brain sections tissue hypoxia and irreversible cell damage were quantitatively determined. Furthermore, we studied hypoxia-related gene expression (VEGF, flt-1). Early treatment significantly (p",

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author = "Seiffge, {David J.} and Lapina, {Natalia E.} and Charalambos Tsagogiorgas and Bastian Theisinger and Henning, {Robert H.} and Lothar Schilling",

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doi = "10.1016/j.expneuro1.2012.06.007",

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Seiffge, DJ, Lapina, NE, Tsagogiorgas, C, Theisinger, B, Henning, RH & Schilling, L 2012, 'Improvement of oxygen supply by an artificial carrier in combination with normobaric oxygenation decreases the volume of tissue hypoxia and tissue damage from transient focal cerebral ischemia', Experimental Neurology, vol. 237, no. 1, pp. 18-25. https://doi.org/10.1016/j.expneuro1.2012.06.007

Improvement of oxygen supply by an artificial carrier in combination with normobaric oxygenation decreases the volume of tissue hypoxia and tissue damage from transient focal cerebral ischemia. / Seiffge, David J.; Lapina, Natalia E.; Tsagogiorgas, Charalambos et al.
In: Experimental Neurology, Vol. 237, No. 1, 09.2012, p. 18-25.

Research output: Contribution to journal › Article › Academic › peer-review

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AU - Theisinger, Bastian

AU - Henning, Robert H.

AU - Schilling, Lothar

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KW - PERFLUOROCARBON EMULSIONS

KW - HYPERBARIC OXYGENATION

KW - REPERFUSION

KW - EXPRESSION

KW - MODEL

KW - PERFUSION

KW - THERAPY

KW - FLUOSOL

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DO - 10.1016/j.expneuro1.2012.06.007

M3 - Article

SN - 0014-4886

VL - 237

SP - 18

EP - 25

JO - Experimental Neurology

JF - Experimental Neurology

IS - 1

ER -

Improvement of oxygen supply by an artificial carrier in combination with normobaric oxygenation decreases the volume of tissue hypoxia and tissue damage from transient focal cerebral ischemia

Abstract

Keywords

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