In childhood acute lymphoblastic leukemia, blasts at different stages of immunophenotypic maturation have stem cell properties

Christoph le Viseur; Marc Hotfilder; Simon Bomken; Kerrie Wilson; Silja Roettgers; Andre Schrauder; Annegret Rosemann; Julie Irving; Ronald W. Stam; Leonard D. Shultz; Jochen Harbott; Heribert Juergens; Martin Schrappe; Rob Pieters; Josef Vormoor

doi:10.1016/j.ccr.2008.05.015

In childhood acute lymphoblastic leukemia, blasts at different stages of immunophenotypic maturation have stem cell properties

Christoph le Viseur
, Marc Hotfilder
, Simon Bomken
, Kerrie Wilson
, Silja Roettgers
, Andre Schrauder
, Annegret Rosemann
, Julie Irving
, Ronald W. Stam
, Leonard D. Shultz
, Jochen Harbott
, Heribert Juergens
, Martin Schrappe
, Rob Pieters
, Josef Vormoor^*

^*Corresponding author for this work

University of Groningen

Research output: Contribution to journal › Article › Academic › peer-review

241 Citations (Scopus)

Abstract

We examined the leukemic stem cell potential of blasts at different stages of maturation in childhood acute lymphoblastic leukemia (ALL). Human leukemic bone marrow was transplanted intrafemorally into NOD/scid mice. Cells sorted using the B precursor differentiation markers CD19, CD20, and CD34 were isolated from patient samples and engrafted mice before serial transplantation into primary or subsequent (up to quaternary) recipients. Surprisingly, blasts representative of all of the different maturational stages were able to reconstitute and reestablish the complete leukemic phenotype in vivo. Sorted blast populations mirrored normal B precursor cells with transcription of a number of stage-appropriate genes. These observations inform a model for leukemia-propagating stem cells in childhood ALL.

Original language	English
Pages (from-to)	47-58
Number of pages	12
Journal	Cancer cell
Volume	14
Issue number	1
DOIs	https://doi.org/10.1016/j.ccr.2008.05.015
Publication status	Published - Jul-2008

Keywords

ACUTE MYELOID-LEUKEMIA
PRIMITIVE HEMATOPOIETIC-CELL
MINIMAL RESIDUAL DISEASE
HUMAN PRECURSOR-B
NOD-SCID MICE
INTRAFEMORAL TRANSPLANTATION
EXPRESSION
CHILDREN
GROWTH
IG

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le Viseur, C., Hotfilder, M., Bomken, S., Wilson, K., Roettgers, S., Schrauder, A., Rosemann, A., Irving, J., Stam, R. W., Shultz, L. D., Harbott, J., Juergens, H., Schrappe, M., Pieters, R., & Vormoor, J. (2008). In childhood acute lymphoblastic leukemia, blasts at different stages of immunophenotypic maturation have stem cell properties. Cancer cell, 14(1), 47-58. https://doi.org/10.1016/j.ccr.2008.05.015

@article{0fb07f2b405943ab936534093161bc8c,

title = "In childhood acute lymphoblastic leukemia, blasts at different stages of immunophenotypic maturation have stem cell properties",

abstract = "We examined the leukemic stem cell potential of blasts at different stages of maturation in childhood acute lymphoblastic leukemia (ALL). Human leukemic bone marrow was transplanted intrafemorally into NOD/scid mice. Cells sorted using the B precursor differentiation markers CD19, CD20, and CD34 were isolated from patient samples and engrafted mice before serial transplantation into primary or subsequent (up to quaternary) recipients. Surprisingly, blasts representative of all of the different maturational stages were able to reconstitute and reestablish the complete leukemic phenotype in vivo. Sorted blast populations mirrored normal B precursor cells with transcription of a number of stage-appropriate genes. These observations inform a model for leukemia-propagating stem cells in childhood ALL.",

keywords = "ACUTE MYELOID-LEUKEMIA, PRIMITIVE HEMATOPOIETIC-CELL, MINIMAL RESIDUAL DISEASE, HUMAN PRECURSOR-B, NOD-SCID MICE, INTRAFEMORAL TRANSPLANTATION, EXPRESSION, CHILDREN, GROWTH, IG",

author = "\{le Viseur\}, Christoph and Marc Hotfilder and Simon Bomken and Kerrie Wilson and Silja Roettgers and Andre Schrauder and Annegret Rosemann and Julie Irving and Stam, \{Ronald W.\} and Shultz, \{Leonard D.\} and Jochen Harbott and Heribert Juergens and Martin Schrappe and Rob Pieters and Josef Vormoor",

year = "2008",

month = jul,

doi = "10.1016/j.ccr.2008.05.015",

language = "English",

volume = "14",

pages = "47--58",

journal = "Cancer cell",

issn = "1535-6108",

publisher = "Cell Press",

number = "1",

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le Viseur, C, Hotfilder, M, Bomken, S, Wilson, K, Roettgers, S, Schrauder, A, Rosemann, A, Irving, J, Stam, RW, Shultz, LD, Harbott, J, Juergens, H, Schrappe, M, Pieters, R & Vormoor, J 2008, 'In childhood acute lymphoblastic leukemia, blasts at different stages of immunophenotypic maturation have stem cell properties', Cancer cell, vol. 14, no. 1, pp. 47-58. https://doi.org/10.1016/j.ccr.2008.05.015

TY - JOUR

T1 - In childhood acute lymphoblastic leukemia, blasts at different stages of immunophenotypic maturation have stem cell properties

AU - le Viseur, Christoph

AU - Hotfilder, Marc

AU - Bomken, Simon

AU - Wilson, Kerrie

AU - Roettgers, Silja

AU - Schrauder, Andre

AU - Rosemann, Annegret

AU - Irving, Julie

AU - Stam, Ronald W.

AU - Shultz, Leonard D.

AU - Harbott, Jochen

AU - Juergens, Heribert

AU - Schrappe, Martin

AU - Pieters, Rob

AU - Vormoor, Josef

PY - 2008/7

Y1 - 2008/7

N2 - We examined the leukemic stem cell potential of blasts at different stages of maturation in childhood acute lymphoblastic leukemia (ALL). Human leukemic bone marrow was transplanted intrafemorally into NOD/scid mice. Cells sorted using the B precursor differentiation markers CD19, CD20, and CD34 were isolated from patient samples and engrafted mice before serial transplantation into primary or subsequent (up to quaternary) recipients. Surprisingly, blasts representative of all of the different maturational stages were able to reconstitute and reestablish the complete leukemic phenotype in vivo. Sorted blast populations mirrored normal B precursor cells with transcription of a number of stage-appropriate genes. These observations inform a model for leukemia-propagating stem cells in childhood ALL.

AB - We examined the leukemic stem cell potential of blasts at different stages of maturation in childhood acute lymphoblastic leukemia (ALL). Human leukemic bone marrow was transplanted intrafemorally into NOD/scid mice. Cells sorted using the B precursor differentiation markers CD19, CD20, and CD34 were isolated from patient samples and engrafted mice before serial transplantation into primary or subsequent (up to quaternary) recipients. Surprisingly, blasts representative of all of the different maturational stages were able to reconstitute and reestablish the complete leukemic phenotype in vivo. Sorted blast populations mirrored normal B precursor cells with transcription of a number of stage-appropriate genes. These observations inform a model for leukemia-propagating stem cells in childhood ALL.

KW - ACUTE MYELOID-LEUKEMIA

KW - PRIMITIVE HEMATOPOIETIC-CELL

KW - MINIMAL RESIDUAL DISEASE

KW - HUMAN PRECURSOR-B

KW - NOD-SCID MICE

KW - INTRAFEMORAL TRANSPLANTATION

KW - EXPRESSION

KW - CHILDREN

KW - GROWTH

KW - IG

U2 - 10.1016/j.ccr.2008.05.015

DO - 10.1016/j.ccr.2008.05.015

M3 - Article

SN - 1535-6108

VL - 14

SP - 47

EP - 58

JO - Cancer cell

JF - Cancer cell

IS - 1

ER -

In childhood acute lymphoblastic leukemia, blasts at different stages of immunophenotypic maturation have stem cell properties

Abstract

Keywords

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