In vitro approaches for the evaluation of human vaccines

Aurora Signorazzi

    Research output: ThesisThesis fully internal (DIV)

    437 Downloads (Pure)

    Abstract

    For many vaccines currently available, every newly produced batch has to be tested in vivo – on animals – to verify its potency. To avoid animal suffering and reduce the variability associated with these tests, we developed assays that could assess the vaccine quality in vitro – using cells.
    We showed that these assays can distinguish vaccine batches of high and low quality. The cell-based tests can therefore be used to approve the commercial use of a batch without animal testing. The methods we developed are also highly informative: they allowed us to understand the mechanism through which the investigated vaccines activate the human immune system. A better understanding of the immunological basis of protection can lead us to better vaccines.
    These in vitro approaches are therefore beneficial in multiple ways, since they can create a vaccine development and testing environment that is both more effective for humans and less harmful to animals.
    Original languageEnglish
    QualificationDoctor of Philosophy
    Awarding Institution
    • University of Groningen
    Supervisors/Advisors
    • Huckriede, Anke, Supervisor
    • Rodenhuis-Zybert, Izabela, Co-supervisor
    Award date19-May-2021
    Place of Publication[Groningen]
    Publisher
    DOIs
    Publication statusPublished - 2021

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