In vitro regeneration of wild chervil (Anthriscus sylvestris L.)

Oktavia Hendrawati, Jacques Hille, Herman J. Woerdenbag*, Wim J. Quax, Oliver Kayser

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

8 Citations (Scopus)
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Abstract

Anthriscus sylvestris (L.) Hoffm. (Apiaceae) is a common wild plant that accumulates the lignan deoxypodophyllotoxin. Deoxypodophyllotoxin can be hydroxylated at the C-7 position in recombinant organisms yielding podophyllotoxin, which is used as a semi-synthetic precursor for the anticancer drugs, etoposide phosphate and teniposide. As in vitro regeneration of A. sylvestris has not yet been reported, development of a regeneration protocol for A. sylvestris would be useful as a micropropagation tool and for metabolic engineering of the plant. Calli were induced from hypocotyl explants and transferred to shoot induction medium containing zeatin riboside. Regenerated shoots were obtained within 6 mo and were transferred onto growth regulator-free root induction medium containing 1% sucrose. Regenerated plants transferred to soil and acclimatized in a greenhouse. Plants were transferred to the field with a 100% survival rate. Regenerated plants flowered and were fully fertile. This is the first report of complete regeneration of A. sylvestris via shoot organogenesis from callus.
Original languageEnglish
Pages (from-to)355-361
Number of pages7
JournalIn vitro cellular & developmental biology-Plant
Volume48
Issue number3
DOIs
Publication statusPublished - Jun-2012

Keywords

  • Organogenesis
  • Micropropagation
  • Regeneration
  • Tissue culture
  • Deoxypodophyllotoxin
  • AGROBACTERIUM-RHIZOGENES
  • GENETIC-TRANSFORMATION
  • SOMATIC EMBRYOGENESIS
  • SUSPENSION CULTURES
  • AROMATIC PLANTS
  • BELGIAN-ENDIVE
  • CELLS
  • TUMEFACIENS
  • STRATEGIES
  • PHENOTYPE

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