In vivo significance of the G2 restriction point

Floris Foijer, Elly Delzenne-Goette, Marleen Dekker, Hein Te Riele

Research output: Contribution to journalArticleAcademicpeer-review

7 Citations (Scopus)

Abstract

Loss of activity of the retinoblastoma pathway is a common event in human cancer. Mouse models have revealed that tumorigenesis by loss of Rb was accelerated by concomitant loss of the cell cycle inhibitor p27KIP1. This has been attributed to reduced apoptosis and weakening of the G1 checkpoint. However, the role of p27KIP1 in a recently identified G2 restriction point may offer an alternative explanation for this synergy. Here, we have investigated the significance of the G2 restriction point in Rb-deficient pituitaries. We show that Rb loss in the pituitary gland activated the G2 restriction point, as evidenced by the appearance of cyclin B1-p27KIP1 complexes. Somewhat unexpectedly, these complexes remained present in Rb-deficient tumors. These results indicate that the G2 restriction point does operate in vivo. However, in the pituitary gland, this mechanism seems to retard rather than to prevent tumor growth.

Original languageEnglish
Pages (from-to)9244-9247
Number of pages4
JournalCancer Research
Volume67
Issue number19
DOIs
Publication statusPublished - 1-Oct-2007

Keywords

  • Animals
  • Cell Transformation, Neoplastic
  • Cyclin B
  • Cyclin B1
  • Cyclin-Dependent Kinase Inhibitor p27
  • G2 Phase
  • Mice
  • Pituitary Gland
  • Pituitary Neoplasms
  • Retinoblastoma Protein

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