Induction of Bile Canaliculi-Forming Hepatocytes from Human Pluripotent Stem Cells

Lavinija Matakovic, Arend W. Overeem, Karin Klappe, Sven C.D. van IJzendoorn*

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingChapterAcademicpeer-review

1 Citation (Scopus)

Abstract

Cell polarity and formation of bile canaliculi can be achieved in hepatocytes which are generated from patient-derived induced pluripotent stem cells. This allows for the study of endogenous mutant proteins, patient-specific pathogenesis, and drug responses for diseases where hepatocyte polarity and bile canaliculi play a key role. Here, we describe a step-by-step protocol for the generation of bile canaliculi-forming hepatocytes from induced pluripotent stem cells and their evaluation.

Original languageEnglish
Title of host publicationHepatocytes
Subtitle of host publicationMethods and Protocols
EditorsNaoki Tanimizu
Place of PublicationNew York, NY
PublisherHumana Press
Pages71-82
Number of pages12
ISBN (Electronic)978-1-0716-2557-6
ISBN (Print)978-1-0716-2556-9
DOIs
Publication statusPublished - 21-Sept-2022

Publication series

NameMethods in Molecular Biology
Volume2544
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

Keywords

  • Bile canaliculus
  • Hepatocyte
  • Hepatocyte polarity
  • Induced pluripotent stem cell
  • Inherited liver disease

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