Induction of cytotoxic T lymphocyte activity by fusion-active peptide-containing virosomes

A Arkema, A Huckriede, P Schoen, J Wilschut*, T Daemen

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

68 Citations (Scopus)

Abstract

Priming of cytotoxic T lymphocyte (CTL) activity with exogenous antigen requires introduction of the antigen into the MHC class I presentation pathway of antigen-presenting cells. In the present study, we used fusogenic reconstituted envelopes (virosomes), derived from influenza virus, as a carrier system for delivery of a synthetic soluble peptide corresponding to a major murine CTL epitope of the influenza virus nucleoprotein (NP). Virosomes containing encapsulated NP-peptide efficiently sensitized target cells for recognition by influenza-specific CTLs generated through priming of mice with infectious virus. Intramuscular immunization of mice with peptide-containing virosomes induced a potent class I MHC-restricted CTL response against influenza-infected target cells. By contrast, an equal dose of NP-peptide encapsulated in fusion-inactivated virosomes did not induce CTL activity, indicating an essential role of the membrane fusion activity of the virosomes in the induction of the response. Likewise, NP-peptide encapsulated in liposomes, NP-peptide mixed with empty virosomes and NP-peptide in IFA failed to induce a CTL response. These results demonstrate that fusion-active virosomes represent a promising delivery system for induction of class I MHC-restricted CTL activity with non-replicating viral antigens. (C) 2000 Elsevier Science Ltd. All rights reserved.

Original languageEnglish
Pages (from-to)1327-1333
Number of pages7
JournalVaccine
Volume18
Issue number14
Publication statusPublished - 31-Jan-2000

Keywords

  • virosome
  • antigen processing
  • peptide immunization
  • INFLUENZA-VIRUS ENVELOPES
  • MAJOR HISTOCOMPATIBILITY COMPLEX
  • CLASS-I PRESENTATION
  • EXOGENOUS ANTIGENS
  • MEMBRANE-FUSION
  • MHC MOLECULES
  • CELLS
  • VACCINATION
  • MACROPHAGES
  • HEMAGGLUTININ

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