INDUCTION OF INTERLEUKIN-1-BETA MESSENGER-RNA AFTER FOCAL CEREBRAL-ISCHEMIA IN THE RAT

M BUTTINI, A SAUTER, HWGM BODDEKE

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Abstract

The expression of interleukin-1beta (IL-1beta) mRNA in the brain in response to cerebral ischaemia in rats was examined using in situ hybridization histochemistry. Focal cerebral ischaemia was induced in spontaneously hypertensive rats by permanent occlusion of the left middle cerebral artery (MCAO). Whereas no IL-1beta mRNA could be detected in non-operated and sham-operated rats, middle cerebral artery occlusion induced the expression of IL-1beta mRNA within 15 min in the ischaemic brain regions prone to become necrotic after 1-2 days. The message appeared as spot-like signals, reached a peak after 3 h and then declined to undetectable levels within 4 days. Additionally, a pronounced but brief induction of IL-1beta mRNA could be detected 1 h after MCAO in the meninges near the watershed zone. The results demonstrate that the inflammatory cytokine IL-1beta is induced in a time-dependent way after brain ischaemia.

Original languageEnglish
Pages (from-to)126-134
Number of pages9
JournalMolecular Brain Research
Volume23
Issue number1-2
Publication statusPublished - Apr-1994

Keywords

  • INTERLEUKIN-1-BETA
  • MIDDLE CEREBRAL ARTERY OCCLUSION
  • BRAIN ISCHEMIA
  • MESSENGER RNA
  • IN-SITU HYBRIDIZATION
  • CORTICOTROPIN-RELEASING FACTOR
  • CENTRAL NERVOUS-SYSTEM
  • BRAIN INTERLEUKIN-1
  • AMEBOID MICROGLIA
  • ARTERY OCCLUSION
  • GENE-EXPRESSION
  • MACROPHAGES
  • CYTOKINES
  • RECEPTOR
  • INJURY

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