INFLUENCE OF AGING ON ANTIBODY-FORMATION INVIVO AFTER IMMUNIZATION WITH THE PRIMARY T-CELL DEPENDENT ANTIGEN HELIX-POMATIA HEMOCYANIN

GE DEGREEF, CGM KALLENBERG, GJ VANSTAALDUINEN, EJ REMARQUE, YI TJANDRA, W HIJMANS

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Abstract

The in vivo antibody response to the primary T-cell dependent antigen Helix pomatia Haemocyanin (HPH) was studied, in order to detect the possible presence of a humoral immune deficiency in ageing. The IgG subclass distribution of the specific antibodies was also determined. In order to define a dose of HPH which could be used to discriminate between the responsiveness of healthy and immunocompromised individuals, we first established a dose-response curve for this antigen in 60 healthy young volunteers. Their responses were compared with the responses of a group of patients suffering from end stage renal failure. The patients who were treated with haemodialysis showed a significantly lower IgM, IgG and IgA anti-HPH antibody response after immunisation with a dose of 30 mug HPH, which could be restored by increasing the antigen dose. Patients treated with continuous ambulant peritoneal dialysis and a group of elderly persons, selected according to the Senieur protocol, showed no impairment of antibody formation after immunisation with 30 mug HPH, but in the non-Senieur elderly the anti-HPH antibody response was significantly lower. Furthermore, Senieur and non-Senieur elderly persons showed a diminished IgG2 anti-HPH antibody formation, whereas in the elderly non-Senieur individuals and in the patients with renal insufficiency, IgG1 and IgG3 anti-HPH antibodies were also diminished. This study clearly shows that the so-called age-associated immune deficiency can be the result of disease and is not necessarily due to the ageing process itself.

Original languageEnglish
Pages (from-to)15 - 28
Number of pages14
JournalMechanisms of Ageing and Development
Volume66
Issue number1
DOIs
Publication statusPublished - Oct-1992

Keywords

  • AGING
  • SENIEUR
  • HELIX-POMATIA HEMOCYANIN
  • ANTIBODIES
  • CAPD
  • HEMODIALYSIS
  • HEPATITIS-B VACCINE
  • KEYHOLE LIMPET HEMOCYANIN
  • IMMUNE-RESPONSE
  • IGG SUBCLASSES
  • HEMODIALYSIS-PATIENTS
  • IMMUNIZATION
  • INVITRO
  • SERUM
  • GLOMERULONEPHRITIS
  • IMMUNOGENICITY

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