Deposition efficiencies of six coagulase-negative staphylococcal strains in phosphate buffered saline (pH 7) to positively (PMMA/TMAEMA-Cl) and negatively (PMMA) charged collector surfaces were studied using a parallel plate flow chamber and "real-time" image analysis. The influence of hydrophobic interactions on the deposition process was kept the same for all combinations of strains and polymers by choosing strains and polymers with similar water contact angles. Zeta potentials of the strains ranged from -14.5 to +4.5 mV and zeta potentials for the collector surfaces were -13 mV for PMMA and + 2 mV for PMMA/TMAEMA-Cl. Deposition efficiencies were expressed as the ratios between the experimentally observed initial deposition rates and the initial deposition rates, calculated on the basis of the Smoluchowski-Levich approximation. In case of the negatively charged PMMA collectors, deposition efficiencies decreased with increasing electrostatic repulsion between bacteria and the collector, as theoretically expected. However, in case of the positively charged PMMA/TMAEMA-Cl collectors, no such relation was found. This may be due to the fact that the surface charge of this material easily change in time after contact with buffer. Another reason may be the high mobility of the molecules, easily reorienting themselves when contacted with different interfaces, like a fluid or a cell interface.
|Number of pages||12|
|Journal||Journal of dispersion science and technology|
|Publication status||Published - 1992|
|Event||SYMP ON THE INTERACTION OF BIOLOGICAL COMPONENTS WITH INTERFACES, AT THE 201ST NATIONAL MEETING OF THE AMERICAN CHEM SOC - , Gabon|
Duration: 1-Apr-1991 → …
- METHACRYLATE POLYMERS