Influence of the relative humidity on the morphology of inkjet printed spots of IgG on a non-porous substrate

Liyakat Hamid Mujawar, J. G. M. Kuerten, D. P. Siregar, Aart van Amerongen*, Willem Norde

*Corresponding author for this work

    Research output: Contribution to journalArticleAcademicpeer-review

    9 Citations (Scopus)
    45 Downloads (Pure)

    Abstract

    During the drying of inkjet printed droplets, the solute particles (IgG-Alexa-635 molecules) in the drop may distribute unevenly on the substrate, resulting in a "coffee-stain" spot morphology. In our study, we investigated the influence of the relative humidity on the distribution of inkjet printed fluorophore labeled IgG molecules on a polystyrene substrate. A theoretical model for an evaporating droplet was developed in order to predict the changes in the spot diameter, height and volume of a drying droplet. An experiment was performed where a sessile droplet was monitored using a CCD camera installed on a goniometer and good agreement was found between the experimental results and simulation data. We also compared the predicted morphology for an inkjet-printed microarray spot with the experimental results where IgG molecules were printed for various relative humidities. The spot morphology of the dried spots was analyzed by a confocal laser microscopy. At a lower relative humidity (i.e.,

    Original languageEnglish
    Pages (from-to)19380-19388
    Number of pages9
    JournalRSC Advances
    Volume4
    Issue number37
    DOIs
    Publication statusPublished - 29-Apr-2014

    Keywords

    • EXPERIMENTAL-VERIFICATION
    • PROTEIN MICROARRAYS
    • DNA MICROARRAYS
    • CONTACT-ANGLE
    • EVAPORATION
    • SURFACES
    • DROPS
    • PERFORMANCE
    • FABRICATION
    • ADSORPTION

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