Inhibition of carbonic anhydrase in dog plasma

P. Rispens; J. Hessels; A. Zwart; W. G.  Zijlstra

doi:10.1007/BF00589244

Inhibition of carbonic anhydrase in dog plasma

P. Rispens, J. Hessels, A. Zwart, W. G. Zijlstra^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

12 Citations (Scopus)

Abstract

Using a pH stat method, we measured the activity of carbonic anhydrase (CA) from dog erythrocytes in the presence of various amounts of dog plasma. A plasma factor appeared to be able to inhibit about 86% of the total CA activity, corresponding to the relative activity of CA II. Naiodoacetate was shown to inhibit the total CA activity up to about 13%, corresponding to the relative activity of CA I. Cl− inhibited the total CA activity up to about 20%, presumably mainly through its strong influence on Ca I. It is concluded that with a degree of haemolysis of up to 3%, no appreciable plasma CA activity will occur.

Original language	English
Pages (from-to)	344-347
Number of pages	4
Journal	Pflugers archiv-European journal of physiology
Volume	403
Issue number	4
DOIs	https://doi.org/10.1007/BF00589244
Publication status	Published - 1985

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title = "Inhibition of carbonic anhydrase in dog plasma",

abstract = "Using a pH stat method, we measured the activity of carbonic anhydrase (CA) from dog erythrocytes in the presence of various amounts of dog plasma. A plasma factor appeared to be able to inhibit about 86% of the total CA activity, corresponding to the relative activity of CA II. Naiodoacetate was shown to inhibit the total CA activity up to about 13%, corresponding to the relative activity of CA I. Cl− inhibited the total CA activity up to about 20%, presumably mainly through its strong influence on Ca I. It is concluded that with a degree of haemolysis of up to 3%, no appreciable plasma CA activity will occur.",

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AU - Zwart, A.

AU - Zijlstra, W. G.

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N2 - Using a pH stat method, we measured the activity of carbonic anhydrase (CA) from dog erythrocytes in the presence of various amounts of dog plasma. A plasma factor appeared to be able to inhibit about 86% of the total CA activity, corresponding to the relative activity of CA II. Naiodoacetate was shown to inhibit the total CA activity up to about 13%, corresponding to the relative activity of CA I. Cl− inhibited the total CA activity up to about 20%, presumably mainly through its strong influence on Ca I. It is concluded that with a degree of haemolysis of up to 3%, no appreciable plasma CA activity will occur.

AB - Using a pH stat method, we measured the activity of carbonic anhydrase (CA) from dog erythrocytes in the presence of various amounts of dog plasma. A plasma factor appeared to be able to inhibit about 86% of the total CA activity, corresponding to the relative activity of CA II. Naiodoacetate was shown to inhibit the total CA activity up to about 13%, corresponding to the relative activity of CA I. Cl− inhibited the total CA activity up to about 20%, presumably mainly through its strong influence on Ca I. It is concluded that with a degree of haemolysis of up to 3%, no appreciable plasma CA activity will occur.

U2 - 10.1007/BF00589244

DO - 10.1007/BF00589244

M3 - Article

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JO - Pflugers archiv-European journal of physiology

JF - Pflugers archiv-European journal of physiology

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