Inhibition of complement factor C5 protects against anti-myeloperoxidase antibody-mediated glomerulonephritis in mice

D. Huugen, A. van Esch, H. Xiao, C. J. Peutz-Kootstra, W. A. Buurman, J. W. Cohen Tervaert, J. C. Jennette, P. Heeringa*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

171 Citations (Scopus)

Abstract

In mice, administration of murine anti-myeloperoxidase (MPO) IgG induces pauci-immune necrotizing crescentic glomerulonephritis. Recent studies in this model indicate a crucial role for complement activation in disease induction. Here, we investigated the effect of pretreatment or intervention with a C5- inhibiting monoclonal antibody (BB5.1) in the mouse model of anti-MPO IgG-induced glomerulonephritis. Mice received BB5.1 8 h before or 1 day after disease induction with anti-MPO IgG and lipopolysaccharide. Mice were killed after 1 or 7 days. Control antibody-pretreated mice developed hematuria, leukocyturia and albuminuria, and glomerulonephritis with a mean of 21.0 +/- 8.8% glomerular crescents and 12.8 +/- 5.5% glomerular capillary necrosis. BB5.1 pretreatment prevented disease development, as evidenced by the absence of urinary abnormalities, a marked reduction in glomerular neutrophil influx at day 1 and normal renal morphology at day 7. Importantly, BB5.1 administration 1 day after disease induction also resulted in a marked attenuation of urinary abnormalities and a more than 80% reduction in glomerular crescent formation. In conclusion, inhibition of C5 activation attenuates disease development in a mouse model of anti MPO IgG-induced glomerulonephritis. These results favor further investigations into the role of complement activation in human MPO-anti-neutrophil cytoplasmic autoantibody-mediated glomerulonephritis, and indicate that inhibition of C5 activation is a potential therapeutic approach in this disease.

Original languageEnglish
Pages (from-to)646-654
Number of pages9
JournalKidney International
Volume71
Issue number7
DOIs
Publication statusPublished - Apr-2007

Keywords

  • anti-neutrophil cytoplasmic autoantibody
  • glomerulonephritis
  • neutrophil
  • complement
  • animal model
  • ANTINEUTROPHIL CYTOPLASMIC AUTOANTIBODIES
  • CRESCENTIC GLOMERULONEPHRITIS
  • GLOMERULAR DEPOSITION
  • IN-VITRO
  • MYELOPEROXIDASE
  • NEUTROPHILS
  • VASCULITIS
  • ACTIVATION
  • ANCA
  • ADHESION

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