Insight into polyhydroxyalkanoate (PHA) production from xylose and extracellular PHA degradation by a thermophilic Schlegelella thermodepolymerans

Wen Zhou, Dana Irene Colpa, Hjalmar Permentier, Ruben Ate Offringa, Leon Rohrbach, Gert Jan Willem Euverink, Janneke Krooneman*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

6 Citations (Scopus)
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Abstract

Accumulation of non-degradable plastic waste in the environment might be prevented by the use of biodegradable polyhydroxyalkanoate (PHA). In this study, the thermophile Schlegelella thermodepolymerans produced up to 80 wt% PHA based on dry cell mass. The largest PHA granules were found in the cells within 48 h using 20 g/L xylose, a C/N ratio of 100, an initial pH of 7, at 50 °C. The substrate consumption, pH changes, and cell growth were monitored, revealing the time dependency of PHA production in S. thermodepolymerans. The metabolic pathways from xylose to PHA were identified based on proteomic analysis, revealing involvement of classic phaCAB, de novo fatty acid biosynthesis, and fatty acid β-oxidation. In addition, it was shown that S. thermodepolymerans degraded extracellular PHA with a high efficiency at 50 °C.

Original languageEnglish
Article number107006
JournalResources, Conservation and Recycling
Volume194
DOIs
Publication statusPublished - Jul-2023

Keywords

  • polyhydroxyalkanoate (PHA)
  • Proteomics
  • Schlegelella thermodepolymerans
  • Synthesis and degradation
  • Xylose metabolic pathway

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