TY - JOUR
T1 - Insight into polyhydroxyalkanoate (PHA) production from xylose and extracellular PHA degradation by a thermophilic Schlegelella thermodepolymerans
AU - Zhou, Wen
AU - Colpa, Dana Irene
AU - Permentier, Hjalmar
AU - Offringa, Ruben Ate
AU - Rohrbach, Leon
AU - Euverink, Gert Jan Willem
AU - Krooneman, Janneke
N1 - Funding Information:
W. Zhou thanks the China Scholarship Council (CSC) for providing financial support (CSC, grant number: 201,806,300,116 ). This research was also supported by the Bio Economy Region Northern Netherlands cooperative (BERNN), and has received funding from the European Regional Development Fund (EFRO) and was co-funded by the Northern Netherlands Provinces (SNN) Spatial Economic Program.
Publisher Copyright:
© 2023 The Author(s)
PY - 2023/7
Y1 - 2023/7
N2 - Accumulation of non-degradable plastic waste in the environment might be prevented by the use of biodegradable polyhydroxyalkanoate (PHA). In this study, the thermophile Schlegelella thermodepolymerans produced up to 80 wt% PHA based on dry cell mass. The largest PHA granules were found in the cells within 48 h using 20 g/L xylose, a C/N ratio of 100, an initial pH of 7, at 50 °C. The substrate consumption, pH changes, and cell growth were monitored, revealing the time dependency of PHA production in S. thermodepolymerans. The metabolic pathways from xylose to PHA were identified based on proteomic analysis, revealing involvement of classic phaCAB, de novo fatty acid biosynthesis, and fatty acid β-oxidation. In addition, it was shown that S. thermodepolymerans degraded extracellular PHA with a high efficiency at 50 °C.
AB - Accumulation of non-degradable plastic waste in the environment might be prevented by the use of biodegradable polyhydroxyalkanoate (PHA). In this study, the thermophile Schlegelella thermodepolymerans produced up to 80 wt% PHA based on dry cell mass. The largest PHA granules were found in the cells within 48 h using 20 g/L xylose, a C/N ratio of 100, an initial pH of 7, at 50 °C. The substrate consumption, pH changes, and cell growth were monitored, revealing the time dependency of PHA production in S. thermodepolymerans. The metabolic pathways from xylose to PHA were identified based on proteomic analysis, revealing involvement of classic phaCAB, de novo fatty acid biosynthesis, and fatty acid β-oxidation. In addition, it was shown that S. thermodepolymerans degraded extracellular PHA with a high efficiency at 50 °C.
KW - polyhydroxyalkanoate (PHA)
KW - Proteomics
KW - Schlegelella thermodepolymerans
KW - Synthesis and degradation
KW - Xylose metabolic pathway
UR - http://www.scopus.com/inward/record.url?scp=85153071263&partnerID=8YFLogxK
U2 - 10.1016/j.resconrec.2023.107006
DO - 10.1016/j.resconrec.2023.107006
M3 - Article
AN - SCOPUS:85153071263
SN - 0921-3449
VL - 194
JO - Resources, Conservation and Recycling
JF - Resources, Conservation and Recycling
M1 - 107006
ER -