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Abstract
Energy-coupling factor (ECF)-type transporters are small, asymmetric membrane protein complexes (∼115 kDa) that consist of a membrane-embedded, substrate-binding protein (S component) and a tripartite ATP-hydrolyzing module (ECF module). They import micronutrients into bacterial cells and have been proposed to use a highly unusual transport mechanism, in which the substrate is dragged across the membrane by a toppling motion of the S component. However, it remains unclear how the lipid bilayer could accommodate such a movement. Here, we used cryogenic electron microscopy at 200 kV to determine structures of a folate-specific ECF transporter in lipid nanodiscs and detergent micelles at 2.7- and 3.4-Å resolution, respectively. The structures reveal an irregularly shaped bilayer environment around the membrane-embedded complex and suggest that toppling of the S component is facilitated by protein-induced membrane deformations. In this way, structural remodeling of the lipid bilayer environment is exploited to guide the transport process.
Original language | English |
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Article number | e2105014118 |
Number of pages | 9 |
Journal | Proceedings of the National Academy of Sciences of the United States of America |
Volume | 118 |
Issue number | 34 |
DOIs | |
Publication status | Published - 24-Aug-2021 |
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Dive into the research topics of 'Insights into the bilayer-mediated toppling mechanism of a folate-specific ECF transporter by cryo-EM'. Together they form a unique fingerprint.Datasets
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Cryo-EM structure of the folate-specific ECF transporter complex in MSP2N2 lipid nanodiscs (200 kV)
Thangaratnarajah, C. (Data Collector), Rheinberger, J. (Data Collector), Batista Paulino, C. (Supervisor) & Slotboom, D. (Supervisor), European Bioinformatics Institute (EMBL-EBI), 8-Feb-2022
DOI: 10.6019/EMPIAR-10942
Dataset
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Cryo-EM structure of the folate-specific ECF transporter complex in DDM micelles (200 kV)
Thangaratnarajah, C. (Data Collector), Rheinberger, J. (Data Collector), Batista Paulino, C. (Supervisor) & Slotboom, D. (Supervisor), European Bioinformatics Institute (EMBL-EBI), 7-Feb-2022
DOI: 10.6019/EMPIAR-10941
Dataset
Projects
- 1 Finished
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oLife Fellowship Programme
Roos, W. (PI), van der Tak, F. (PI), Zijlstra, W. (PI), Dobos, V. (Postdoc), Heinen, L. (Postdoc), Thangaratnarajah, C. (Postdoc), Hoekzema, M. (Postdoc), Blokhuis, A. (Postdoc), Mascotti, L. (Postdoc), Padin Santos, D. (Postdoc), Chopra, A. (Postdoc), Obermaier, S. (Postdoc), Driver, M. (Postdoc), Moreira Goulart, M. (Postdoc), Sasidharan, S. (Postdoc), Samar Mahapatra, S. (Postdoc), Zylstra, A. (Postdoc), Geiger, Y. (Postdoc), Llopis Lorente, A. (Postdoc), Aschmann, D. (Postdoc) & Kulala Vittala, S. (Postdoc)
01/04/2019 → 31/03/2024
Project: Research