TY - JOUR
T1 - Integrated proteogenomic approach identifying a protein signature of COPD and a new splice variant of SORBS1
AU - Brandsma, Corry-Anke
AU - Guryev, Victor
AU - Timens, Wim
AU - Ciconelle, Ana
AU - Postma, Dirkje S
AU - Bischoff, Rainer
AU - Johansson, Maria
AU - Ovchinnikova, Ekaterina S
AU - Malm, Johan
AU - Marko-Varga, Gyorgy
AU - Fehniger, Thomas E
AU - van den Berge, Maarten
AU - Horvatovich, Peter
N1 - © Author(s) (or their employer(s)) 2020. Re-use permitted under CC BY. Published by BMJ.
PY - 2020/2
Y1 - 2020/2
N2 - Translation of genomic alterations to protein changes in chronic obstructive pulmonary disease (COPD) is largely unexplored. Using integrated proteomic and RNA sequencing analysis of COPD and control lung tissues, we identified a protein signature in COPD characterised by extracellular matrix changes and a potential regulatory role for SUMO2. Furthermore, we identified 61 differentially expressed novel, non-reference, peptides in COPD compared with control lungs. This included two peptides encoding for a new splice variant of SORBS1, of which the transcript usage was higher in COPD compared with control lungs. These explorative findings and integrative proteogenomic approach open new avenues to further unravel the pathology of COPD.
AB - Translation of genomic alterations to protein changes in chronic obstructive pulmonary disease (COPD) is largely unexplored. Using integrated proteomic and RNA sequencing analysis of COPD and control lung tissues, we identified a protein signature in COPD characterised by extracellular matrix changes and a potential regulatory role for SUMO2. Furthermore, we identified 61 differentially expressed novel, non-reference, peptides in COPD compared with control lungs. This included two peptides encoding for a new splice variant of SORBS1, of which the transcript usage was higher in COPD compared with control lungs. These explorative findings and integrative proteogenomic approach open new avenues to further unravel the pathology of COPD.
U2 - 10.1136/thoraxjnl-2019-213200
DO - 10.1136/thoraxjnl-2019-213200
M3 - Article
C2 - 31937552
VL - 75
SP - 180
EP - 183
JO - Thorax
JF - Thorax
SN - 0040-6376
IS - 2
ER -