Interaction between the Adh and αGpdh loci in Drosophila melanogaster: Adult survival at high temperature

L. Oudman; W. van Delden; A. Kamping; R. Bijlsma

doi:10.1038/hdy.1992.43

Interaction between the Adh and αGpdh loci in Drosophila melanogaster: Adult survival at high temperature

L. Oudman^*, W. van Delden, A. Kamping, R. Bijlsma

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

15 Citations (Scopus)

Abstract

The role of high temperature resistance in the world-wide cline of Adh and alpha-Gpdh allele frequencies of Drosophila melanogaster was investigated. Experimental strains were used with different combinations of Adh and alpha-Gpdh alleles but with similar genetic background. The survival time of adult males, reared at different pre-adult temperatures, was measured at 35-degrees-C. To investigate the relationship between survival and protein content, triglycerides content and ADH and alpha-GPDH enzyme activity, the latter characters were measured before and after 12 h exposure to 35-degrees-C. Three-way ANOVA of survival at 35-degrees-C showed significant effects of rearing temperature and Adh genotype, interaction between Adh and alpha-Cpdh, interaction between Adh and rearing temperature and interaction between Adh, alpha-Gpdh and rearing temperature, alpha-GPDH enzyme activity did not change significantly. Although protein content, triglycerides content and ADH enzyme activity decreased significantly at 35-degrees-C, no correlation was observed between survival and these traits. It is concluded that the world-wide cline of Adh and alpha-Gpdh allele frequencies cannot simply be explained by the genotypic differences in resistance to high temperature of adult Drosophila melanogaster.

Original language	English
Pages (from-to)	289-297
Number of pages	9
Journal	Heredity
Volume	68
DOIs	https://doi.org/10.1038/hdy.1992.43
Publication status	Published - Apr-1992

Keywords

ALCOHOL DEHYDROGENASE
ALLOZYME POLYMORPHISM
CLINE
ALPHA-GLYCEROPHOSPHATE DEHYDROGENASE
SELECTION, TEMPERATURE STRESS
ALCOHOL-DEHYDROGENASE LOCUS
SN-GLYCEROL-3-PHOSPHATE DEHYDROGENASE
DESICCATION TOLERANCE
ENZYME POLYMORPHISMS
ETHANOL
STRESS
SELECTION
POPULATIONS
LARVAE

Access to Document

10.1038/hdy.1992.43

Handle.net

Persistent link

Embargoed Document

Interaction between the Adh and αGpdh loci in Drosophila
Final publisher's version, 3.85 MB
Request copy

Cite this

@article{c35c190ddb834652aa67a6ce0aa607d4,

title = "Interaction between the Adh and αGpdh loci in Drosophila melanogaster: Adult survival at high temperature",

abstract = "The role of high temperature resistance in the world-wide cline of Adh and alpha-Gpdh allele frequencies of Drosophila melanogaster was investigated. Experimental strains were used with different combinations of Adh and alpha-Gpdh alleles but with similar genetic background. The survival time of adult males, reared at different pre-adult temperatures, was measured at 35-degrees-C. To investigate the relationship between survival and protein content, triglycerides content and ADH and alpha-GPDH enzyme activity, the latter characters were measured before and after 12 h exposure to 35-degrees-C. Three-way ANOVA of survival at 35-degrees-C showed significant effects of rearing temperature and Adh genotype, interaction between Adh and alpha-Cpdh, interaction between Adh and rearing temperature and interaction between Adh, alpha-Gpdh and rearing temperature, alpha-GPDH enzyme activity did not change significantly. Although protein content, triglycerides content and ADH enzyme activity decreased significantly at 35-degrees-C, no correlation was observed between survival and these traits. It is concluded that the world-wide cline of Adh and alpha-Gpdh allele frequencies cannot simply be explained by the genotypic differences in resistance to high temperature of adult Drosophila melanogaster.",

keywords = "ALCOHOL DEHYDROGENASE, ALLOZYME POLYMORPHISM, CLINE, ALPHA-GLYCEROPHOSPHATE DEHYDROGENASE, SELECTION, TEMPERATURE STRESS, ALCOHOL-DEHYDROGENASE LOCUS, SN-GLYCEROL-3-PHOSPHATE DEHYDROGENASE, DESICCATION TOLERANCE, ENZYME POLYMORPHISMS, ETHANOL, STRESS, SELECTION, POPULATIONS, LARVAE",

author = "L. Oudman and {van Delden}, W. and A. Kamping and R. Bijlsma",

year = "1992",

month = apr,

doi = "10.1038/hdy.1992.43",

language = "English",

volume = "68",

pages = "289--297",

journal = "Heredity",

issn = "0018-067X",

publisher = "Nature Publishing Group",

}

TY - JOUR

T1 - Interaction between the Adh and αGpdh loci in Drosophila melanogaster

T2 - Adult survival at high temperature

AU - Oudman, L.

AU - van Delden, W.

AU - Kamping, A.

AU - Bijlsma, R.

PY - 1992/4

Y1 - 1992/4

N2 - The role of high temperature resistance in the world-wide cline of Adh and alpha-Gpdh allele frequencies of Drosophila melanogaster was investigated. Experimental strains were used with different combinations of Adh and alpha-Gpdh alleles but with similar genetic background. The survival time of adult males, reared at different pre-adult temperatures, was measured at 35-degrees-C. To investigate the relationship between survival and protein content, triglycerides content and ADH and alpha-GPDH enzyme activity, the latter characters were measured before and after 12 h exposure to 35-degrees-C. Three-way ANOVA of survival at 35-degrees-C showed significant effects of rearing temperature and Adh genotype, interaction between Adh and alpha-Cpdh, interaction between Adh and rearing temperature and interaction between Adh, alpha-Gpdh and rearing temperature, alpha-GPDH enzyme activity did not change significantly. Although protein content, triglycerides content and ADH enzyme activity decreased significantly at 35-degrees-C, no correlation was observed between survival and these traits. It is concluded that the world-wide cline of Adh and alpha-Gpdh allele frequencies cannot simply be explained by the genotypic differences in resistance to high temperature of adult Drosophila melanogaster.

AB - The role of high temperature resistance in the world-wide cline of Adh and alpha-Gpdh allele frequencies of Drosophila melanogaster was investigated. Experimental strains were used with different combinations of Adh and alpha-Gpdh alleles but with similar genetic background. The survival time of adult males, reared at different pre-adult temperatures, was measured at 35-degrees-C. To investigate the relationship between survival and protein content, triglycerides content and ADH and alpha-GPDH enzyme activity, the latter characters were measured before and after 12 h exposure to 35-degrees-C. Three-way ANOVA of survival at 35-degrees-C showed significant effects of rearing temperature and Adh genotype, interaction between Adh and alpha-Cpdh, interaction between Adh and rearing temperature and interaction between Adh, alpha-Gpdh and rearing temperature, alpha-GPDH enzyme activity did not change significantly. Although protein content, triglycerides content and ADH enzyme activity decreased significantly at 35-degrees-C, no correlation was observed between survival and these traits. It is concluded that the world-wide cline of Adh and alpha-Gpdh allele frequencies cannot simply be explained by the genotypic differences in resistance to high temperature of adult Drosophila melanogaster.

KW - ALCOHOL DEHYDROGENASE

KW - ALLOZYME POLYMORPHISM

KW - CLINE

KW - ALPHA-GLYCEROPHOSPHATE DEHYDROGENASE

KW - SELECTION, TEMPERATURE STRESS

KW - ALCOHOL-DEHYDROGENASE LOCUS

KW - SN-GLYCEROL-3-PHOSPHATE DEHYDROGENASE

KW - DESICCATION TOLERANCE

KW - ENZYME POLYMORPHISMS

KW - ETHANOL

KW - STRESS

KW - SELECTION

KW - POPULATIONS

KW - LARVAE

U2 - 10.1038/hdy.1992.43

DO - 10.1038/hdy.1992.43

M3 - Article

SN - 0018-067X

VL - 68

SP - 289

EP - 297

JO - Heredity

JF - Heredity

ER -

Interaction between the Adh and αGpdh loci in Drosophila melanogaster: Adult survival at high temperature

Abstract

Keywords

Access to Document

Handle.net

Embargoed Document

Fingerprint

Cite this