Interference in initial adhesion of uropathogenic bacteria and yeasts to silicone rubber by a Lactobacillus acidophilus biosurfactant

MMC Velraeds, B van de Belt-Gritter, HC van der Mei*, G Reid, HJ Busscher

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

131 Citations (Scopus)

Abstract

The ability of the Lactobacillus acidophilus RC14 biosurfactant 'surlactin' to inhibit the initial adhesion of various uropathogenic bacteria and two yeast strains to silicone rubber was investigated in a parallel-plate flow chamber in filter-sterilised pooled human urine, A parallel-plate flow chamber with a silicone rubber bottom plate was filled with a 1.0 mg/ml biosurfactant solution for adsorption overnight (18 h), Subsequently, the adhesion of the bacterial or yeast cells from a urine suspension under low how (shear rate 15 s(-1)) was followed in situ by automated image analysis, Control tests were with untreated silicone rubber. Initial deposition rates and numbers of adhering cells after 4 h of dow were determined, Surlactin layers caused a marked inhibition of the initial deposition rates and adhesion numbers after 4 h for the majority of the bacteria (11 of 15 strains tested) and this inhibition was particularly effective against Enterococcus faecalis, Escherichia coli and Staphylococcus epidermidis. Although the initial deposition rates of the two Candida albicans strains were reduced by c. 50% in comparison with the controls, the numbers of yeast cells adhering after 4 h were similar.

Original languageEnglish
Pages (from-to)1081-1085
Number of pages5
JournalJournal of medical microbiology
Volume47
Issue number12
Publication statusPublished - Dec-1998

Keywords

  • URINARY-TRACT INFECTIONS
  • ENTEROCOCCUS-FAECALIS
  • URETHRAL CATHETERS
  • SUBSTRATA
  • RECURRENCE
  • INHIBITION
  • EXCLUSION
  • PROTEIN
  • ESTRIOL
  • WOMEN

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