INTERMOLECULAR INTERACTIONS BETWEEN THE A-SUBUNIT AND B-SUBUNIT OF HEAT-LABILE ENTEROTOXIN FROM ESCHERICHIA-COLI PROMOTE HOLOTOXIN ASSEMBLY AND STABILITY INVIVO

SJ STREATFIELD, M SANDKVIST, TK SIXMA, M BAGDASARIAN, WGJ HOL, TR HIRST*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

34 Citations (Scopus)

Abstract

Cholera toxin and the related heat-labile enterotoxin (LT) produced by Escherichia coli consist of a holotoxin of one A subunit and five B subunits (AB5). Here we investigate the domains of the A subunit (EtxA) of E. coli LT which influence the events of B-subunit (EtxB) oligomerization and the formation of a stable AB5 holotoxin complex. We show that the C-terminal 14 amino acids of the A subunit comprise two functional domains that differentially affect oligomerization and holotoxin stability. Deletion of the last 14 amino acids (-14) from the A subunit resulted in a molecule that was significantly impaired in its capacity to promote the assembly of a mutant B subunit, EtxB191.5. In contrast, deletion of the last four amino acids (-4) from the A subunit gave a molecule that retained such a capacity. This suggests that C-terminal residues within the -14 to -4 region of the A subunit are important for promoting the oligomerization of EtxB. In addition, we demonstrate that the truncated A subunit lacking the last 4 amino acids was unable to form a stable AB5 holotoxin complex even though it promoted B-subunit oligomerization. This suggests that the last 4 residues of the A subunit function as an "anchoring" sequence responsible for maintaining the stability of A/B subunit interaction during holotoxin assembly. These data represent an important example of how intermolecular interactions between polypeptides in vivo can modulate the folding and assembly of a macromolecular complex.

Original languageEnglish
Pages (from-to)12140-12144
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume89
Issue number24
Publication statusPublished - 15-Dec-1992

Keywords

  • PROTEIN EXPORT
  • PROTEIN FOLDING
  • ENDOPLASMIC RETICULUM RETENTION SIGNAL
  • CHOLERA TOXIN
  • PROTEIN-DISULFIDE ISOMERASE
  • NUCLEOTIDE-SEQUENCE
  • CARBOXYL TERMINUS
  • TOXIN GENES
  • IDENTIFICATION
  • DELETION

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