Intrachanges as part of complex chromosome-type exchange aberrations

JJWA Boei*, S Vermeulen, J Moser, LHF Mullenders, AT Natarajan

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

14 Citations (Scopus)

Abstract

The chromosome-type exchange aberrations induced by ionizing radiation during the G(0)/G(1) phase of the cell cycle are believed to be the result of illegitimate rejoining of chromosome breaks. From numerous studies using chromosome painting, it has emerged that even after a moderate dose of radiation, a substantial fraction of these exchanges is complex. Most of them are derived from the free interaction between the ends of three or more breaks. Other studies have demonstrated that chromosomes occupy distinct territories in the interphase nucleus. Since breaks that are in close proximity have an enhanced interaction probability, it seems likely that after ionizing radiation many of the interacting breaks will be present within one chromosome or chromosome arm. Unfortunately, the majority of these intrachanges remain undetected, even when sophisticated molecular cytogenetic detection methods (i.e. mFISH) are applied to paint all chromosome pairs in distinct colors. In the present paper, we evaluate the limitations of full-color painting for the detection of complex exchanges and the correct interpretations of break interactions. (C) 2002 Elsevier Science B.V. All rights reserved.

Original languageEnglish
Pages (from-to)47-55
Number of pages9
JournalMutation research-Fundamental and molecular mechanisms of mutagenesis
Volume504
Issue number1-2
DOIs
Publication statusPublished - 25-Jul-2002
Event5th International Symposium on Chromosomal Aberrations/20th Meeting of the Japanese EMS Mammalian Mutagenicity Study Group - AWAJI ISL, Japan
Duration: 26-Oct-200128-Oct-2001

Keywords

  • ionizing radiation
  • intrachanges
  • complex aberrations
  • proximity effects
  • IN-SITU HYBRIDIZATION
  • TELOMERIC PNA PROBE
  • HUMAN-LYMPHOCYTES
  • INCOMPLETE EXCHANGES
  • DNA PROBES
  • RADIATION
  • FISH
  • COMBINATION

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