Inulin-grown Faecalibacterium prausnitzii cross-feeds fructose to the human intestinal epithelium

Raphael R. Fagundes, Arno R. Bourgonje, Ali Saeed, Arnau Vich Vila, Niels Plomp, Tjasso Blokzijl, Mehdi Sadaghian Sadabad, Julius Z. H. von Martels, Sander S. van Leeuwen, Rinse K. Weersma, Gerard Dijkstra, Hermie J. M. Harmsen, Klaas Nico Faber*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

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Abstract

Many chronic diseases are associated with decreased abundance of the gut commensal Faecalibacterium prausnitzii. This strict anaerobe can grow on dietary fibers, e.g., prebiotics, and produce high levels of butyrate, often associated to epithelial metabolism and health. However, little is known about other F. prausnitzii metabolites that may affect the colonic epithelium. Here, we analyzed prebiotic cross-feeding between F. prausnitzii and intestinal epithelial (Caco-2) cells in a "Human-oxygen Bacteria-anaerobic" coculture system. Inulin-grown F. prausnitzii enhanced Caco-2 viability and suppressed inflammation- and oxidative stress-marker expression. Inulin-grown F. prausnitzii produced excess butyrate and fructose, but only fructose efficiently promoted Caco-2 growth. Finally, fecal microbial taxonomy analysis (16S sequencing) from healthy volunteers (n = 255) showed the strongest positive correlation for F. prausnitzii abundance and stool fructose levels. We show that fructose, produced and accumulated in a fiber-rich colonic environment, supports colonic epithelium growth, while butyrate does not.

Original languageEnglish
Article number1993582
Number of pages18
JournalGut Microbes
Volume13
Issue number1
DOIs
Publication statusPublished - 19-Nov-2021

Keywords

  • Gut bacteria
  • dysbiosis
  • fructose
  • intestinal epithelium
  • Faecalibacterium
  • inulin
  • INFLAMMATORY-BOWEL-DISEASE
  • BUTYRATE-PRODUCING BACTERIA
  • CHAIN FATTY-ACIDS
  • DEGRADATION CAPACITY
  • COLON BACTERIA
  • MICROBIOTA
  • GUT
  • DIVERSITY
  • MUCOSA
  • BIFIDOBACTERIA

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