Isolation and characterization of 11 microsatellite primers for a temperate reef fish, the California sheephead (Semicossyphus pulcher)

M. Poortvliet, J.L. Olsen, K.A Selkoe, J.A. Coyer, Giacomo Bernardi

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Abstract

Eleven microsatellites were characterized for Semicossyphus pulcher (California sheephead) using an enrichment protocol. The number of alleles varied from three to 14 for a sample of 40 individuals from two populations. Expected heterozygosities ranged from 0.311 to 0.891. All loci but one were in Hardy-Weinberg equilibrium. No evidence for linkage disequilibrium was observed. These polymorphic microsatellites will be useful for genetic diversity and connectivity analyses of S. pulcher.

Original languageEnglish
Pages (from-to)429-430
Number of pages2
JournalMolecular Ecology Resources
Volume9
Issue number1
DOIs
Publication statusPublished - Jan-2009

Keywords

  • California sheephead
  • microsatellite
  • population structure
  • Semicossyphus pulcher
  • simple sequence repeat

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