Isolation and partial characterization of a biosurfactant produced by Streptococcus thermophilus A

Ligia R. Rodrigues; Jose A. Teixeira; Henny C. van der Mei; Rosario Oliveira

doi:10.1016/j.colsurfb.2006.08.009

Isolation and partial characterization of a biosurfactant produced by Streptococcus thermophilus A

Ligia R. Rodrigues^*
, Jose A. Teixeira
, Henny C. van der Mei
, Rosario Oliveira

^*Corresponding author for this work

Man, Biomaterials and Microbes (MBM)

Research output: Contribution to journal › Article › Academic › peer-review

125 Citations (Scopus)

Abstract

Isolation and characterization of the surface active components from the crude biosurfactant produced by Streptococcus thermophilus A was studied. A fraction rich in glycolipids was obtained by the fractionation of crude biosurfactant using hydrophobic interaction chromatography. Molecular (by Fourier transform infrared spectroscopy) and elemental compositions (by X-ray photoelectron spectroscopy) were determined. Critical micelle concentration achieved was 20 g/l, allowing for a surface tension value of 36 mJ/m(2). Moreover, this glycolipid rich fraction was found to be an anti-adhesive and antimicrobial agent against several bacterial and yeast strains isolated from explanted voice prostheses. Further purification steps should be carefully analyzed as each purification step will increase the costs and decreases the amounts of biosurfactants recovered. (C) 2006 Elsevier B.V. All rights reserved.

Original language	English
Pages (from-to)	105-112
Number of pages	8
Journal	COLLOIDS AND SURFACES B-BIOINTERFACES
Volume	53
Issue number	1
DOIs	https://doi.org/10.1016/j.colsurfb.2006.08.009
Publication status	Published - 1-Nov-2006

Keywords

biosurfactant
isolation
physicochemical characterization
glycolipid
SUCCINOYL TREHALOSE LIPIDS
SILICONE-RUBBER
VOICE PROSTHESES
PROBIOTIC BACTERIA
BIOFILM FORMATION
IN-VITRO
POTENTIAL APPLICATIONS
LACTOBACILLUS STRAINS
MICROBIAL ADHESION
BACILLUS-SUBTILIS

Access to Document

10.1016/j.colsurfb.2006.08.009

Handle.net

Persistent link

Cite this

@article{59b8f96cbd5a4d2ea42ce6f1622bea68,

title = "Isolation and partial characterization of a biosurfactant produced by Streptococcus thermophilus A",

abstract = "Isolation and characterization of the surface active components from the crude biosurfactant produced by Streptococcus thermophilus A was studied. A fraction rich in glycolipids was obtained by the fractionation of crude biosurfactant using hydrophobic interaction chromatography. Molecular (by Fourier transform infrared spectroscopy) and elemental compositions (by X-ray photoelectron spectroscopy) were determined. Critical micelle concentration achieved was 20 g/l, allowing for a surface tension value of 36 mJ/m(2). Moreover, this glycolipid rich fraction was found to be an anti-adhesive and antimicrobial agent against several bacterial and yeast strains isolated from explanted voice prostheses. Further purification steps should be carefully analyzed as each purification step will increase the costs and decreases the amounts of biosurfactants recovered. (C) 2006 Elsevier B.V. All rights reserved.",

keywords = "biosurfactant, isolation, physicochemical characterization, glycolipid, SUCCINOYL TREHALOSE LIPIDS, SILICONE-RUBBER, VOICE PROSTHESES, PROBIOTIC BACTERIA, BIOFILM FORMATION, IN-VITRO, POTENTIAL APPLICATIONS, LACTOBACILLUS STRAINS, MICROBIAL ADHESION, BACILLUS-SUBTILIS",

author = "Rodrigues, \{Ligia R.\} and Teixeira, \{Jose A.\} and \{van der Mei\}, \{Henny C.\} and Rosario Oliveira",

note = "0 Article J",

year = "2006",

month = nov,

day = "1",

doi = "10.1016/j.colsurfb.2006.08.009",

language = "English",

volume = "53",

pages = "105--112",

journal = "COLLOIDS AND SURFACES B-BIOINTERFACES",

issn = "0927-7765",

publisher = "ELSEVIER SCIENCE BV",

number = "1",

}

TY - JOUR

T1 - Isolation and partial characterization of a biosurfactant produced by Streptococcus thermophilus A

AU - Rodrigues, Ligia R.

AU - Teixeira, Jose A.

AU - van der Mei, Henny C.

AU - Oliveira, Rosario

N1 - 0 Article J

PY - 2006/11/1

Y1 - 2006/11/1

N2 - Isolation and characterization of the surface active components from the crude biosurfactant produced by Streptococcus thermophilus A was studied. A fraction rich in glycolipids was obtained by the fractionation of crude biosurfactant using hydrophobic interaction chromatography. Molecular (by Fourier transform infrared spectroscopy) and elemental compositions (by X-ray photoelectron spectroscopy) were determined. Critical micelle concentration achieved was 20 g/l, allowing for a surface tension value of 36 mJ/m(2). Moreover, this glycolipid rich fraction was found to be an anti-adhesive and antimicrobial agent against several bacterial and yeast strains isolated from explanted voice prostheses. Further purification steps should be carefully analyzed as each purification step will increase the costs and decreases the amounts of biosurfactants recovered. (C) 2006 Elsevier B.V. All rights reserved.

AB - Isolation and characterization of the surface active components from the crude biosurfactant produced by Streptococcus thermophilus A was studied. A fraction rich in glycolipids was obtained by the fractionation of crude biosurfactant using hydrophobic interaction chromatography. Molecular (by Fourier transform infrared spectroscopy) and elemental compositions (by X-ray photoelectron spectroscopy) were determined. Critical micelle concentration achieved was 20 g/l, allowing for a surface tension value of 36 mJ/m(2). Moreover, this glycolipid rich fraction was found to be an anti-adhesive and antimicrobial agent against several bacterial and yeast strains isolated from explanted voice prostheses. Further purification steps should be carefully analyzed as each purification step will increase the costs and decreases the amounts of biosurfactants recovered. (C) 2006 Elsevier B.V. All rights reserved.

KW - biosurfactant

KW - isolation

KW - physicochemical characterization

KW - glycolipid

KW - SUCCINOYL TREHALOSE LIPIDS

KW - SILICONE-RUBBER

KW - VOICE PROSTHESES

KW - PROBIOTIC BACTERIA

KW - BIOFILM FORMATION

KW - IN-VITRO

KW - POTENTIAL APPLICATIONS

KW - LACTOBACILLUS STRAINS

KW - MICROBIAL ADHESION

KW - BACILLUS-SUBTILIS

U2 - 10.1016/j.colsurfb.2006.08.009

DO - 10.1016/j.colsurfb.2006.08.009

M3 - Article

SN - 0927-7765

VL - 53

SP - 105

EP - 112

JO - COLLOIDS AND SURFACES B-BIOINTERFACES

JF - COLLOIDS AND SURFACES B-BIOINTERFACES

IS - 1

ER -

Isolation and partial characterization of a biosurfactant produced by Streptococcus thermophilus A

Abstract

Keywords

Access to Document

Handle.net

Fingerprint

Cite this