Isolation of autophagocytosis mutants of Saccharomyces cerevisiae

M. Thumm, R. Egner, M. Schlumpberger, M. Straub, M. Veenhuis, D.H. Wolf

Research output: Contribution to journalArticleAcademicpeer-review

454 Citations (Scopus)

Abstract

Protein degradation in the vacuole (lysosome) is an important event in cellular regulation. In yeast, as in mammalian cells, a major route of protein uptake for degradation into the vacuole (lysosome) has been found to be autophagocytosis. The discovery of this process in yeast enables the elucidation of its mechanisms via genetic and molecular biological investigations. Here we report the isolation of yeast mutants defective in autophagocytosis (aut mutants), using a rapid colony screening procedure.

Original languageEnglish
Pages (from-to)275-280
Number of pages6
JournalFEBS Letters
Volume349
Issue number2
DOIs
Publication statusPublished - 1-Aug-1994

Keywords

  • YEAST
  • VACUOLE
  • AUTOPHAGOCYTOSIS
  • SCREENING PROCEDURE
  • PROTEINASE-DEFICIENT MUTANTS
  • YEAST VACUOLE LYSOSOME
  • FATTY-ACID SYNTHETASE
  • DEGRADATION
  • PROTEOLYSIS
  • MATURATION
  • AUTOPHAGY
  • YSCB
  • BIOGENESIS
  • CATALYSTS

Cite this