Isolation, sequence and expression in Escherichia coli, Bacillus subtilis and Lactococcus lactis of the DNase (streptodornase)-encoding gene from Streptococcus equisimilis H46A

Renata Wolinowska; Piotr Cegłowski; Jan Kok; Gerhardus Venema

doi:10.1016/0378-1119(91)90574-U

Isolation, sequence and expression in Escherichia coli, Bacillus subtilis and Lactococcus lactis of the DNase (streptodornase)-encoding gene from Streptococcus equisimilis H46A

Renata Wolinowska, Piotr Cegłowski, Jan Kok, Gerhardus Venema

Groningen Biomolecular Sciences and Biotechnology Institute

Research output: Contribution to journal › Comment/Letter to the editor › Academic › peer-review

22 Citations (Scopus)

Abstract

A partial library of BclI-generated chromosomal DNA fragments from Streptococcus equisimilis H64A (Lancefield Group C) was constructed in Escherichia coli. Clones displaying either streptokinase or deoxyribonuclease (streptodornase; SDC) activities were isolated. The gene (sdc) expressing the SDC activity was allocated on the 1.1-kb AccI DNA subfragment. Sequence analysis of this DNA fragment revealed the presence of one open reading frame, which could encode a protein of 36.8 kDa. The N-terminal portion of the deduced protein exhibited features characteristic of prokaryotic signal peptides. The sdc gene was expressed in E. coli, Bacillus subtilis and Lactococcus lactis. As observed for S. equisimilis, in the heterologous Gram+ hosts, at least part of the SDC protein was secreted into the medium.

Original language	English
Pages (from-to)	115-119
Number of pages	5
Journal	Gene
Volume	106
Issue number	1
DOIs	https://doi.org/10.1016/0378-1119(91)90574-U
Publication status	Published - 30-Sept-1991

Keywords

GROUP-C STREPTOCOCCUS
DEOXYRIBONUCLEASE
STREPTOKINASE
RECOMBINANT DNA
SIGNAL PEPTIDE
SECRETION
STREPTOKINASE GENE
NUCLEOTIDE-SEQUENCE
CLONING VECTORS
PLASMID DNA
SYSTEM
CONSTRUCTION
RESISTANCE
CLEAVAGE
PROTEINS
NUCLEASE

Access to Document

10.1016/0378-1119(91)90574-U

Handle.net

Persistent link

Embargoed Document

1991GeneWolinowska.pdf
Final publisher's version, 624 KB
Request copy

Cite this

@article{8c33157be19f40eab03b7020dd0fecf0,

title = "Isolation, sequence and expression in Escherichia coli, Bacillus subtilis and Lactococcus lactis of the DNase (streptodornase)-encoding gene from Streptococcus equisimilis H46A",

abstract = "A partial library of BclI-generated chromosomal DNA fragments from Streptococcus equisimilis H64A (Lancefield Group C) was constructed in Escherichia coli. Clones displaying either streptokinase or deoxyribonuclease (streptodornase; SDC) activities were isolated. The gene (sdc) expressing the SDC activity was allocated on the 1.1-kb AccI DNA subfragment. Sequence analysis of this DNA fragment revealed the presence of one open reading frame, which could encode a protein of 36.8 kDa. The N-terminal portion of the deduced protein exhibited features characteristic of prokaryotic signal peptides. The sdc gene was expressed in E. coli, Bacillus subtilis and Lactococcus lactis. As observed for S. equisimilis, in the heterologous Gram+ hosts, at least part of the SDC protein was secreted into the medium.",

keywords = "GROUP-C STREPTOCOCCUS, DEOXYRIBONUCLEASE, STREPTOKINASE, RECOMBINANT DNA, SIGNAL PEPTIDE, SECRETION, STREPTOKINASE GENE, NUCLEOTIDE-SEQUENCE, CLONING VECTORS, PLASMID DNA, SYSTEM, CONSTRUCTION, RESISTANCE, CLEAVAGE, PROTEINS, NUCLEASE",

author = "Renata Wolinowska and Piotr Ceg{\l}owski and Jan Kok and Gerhardus Venema",

note = "Relation: http://www.rug.nl/gbb/ date\_submitted:2007 Rights: University of Groningen, Groningen Biomolecular Sciences and Biotechnology Institute",

year = "1991",

month = sep,

day = "30",

doi = "10.1016/0378-1119(91)90574-U",

language = "English",

volume = "106",

pages = "115--119",

journal = "Gene",

publisher = "Elsevier",

number = "1",

}

Isolation, sequence and expression in Escherichia coli, Bacillus subtilis and Lactococcus lactis of the DNase (streptodornase)-encoding gene from Streptococcus equisimilis H46A. / Wolinowska, Renata; Cegłowski, Piotr; Kok, Jan et al.
In: Gene, Vol. 106, No. 1, 30.09.1991, p. 115-119.

Research output: Contribution to journal › Comment/Letter to the editor › Academic › peer-review

TY - JOUR

T1 - Isolation, sequence and expression in Escherichia coli, Bacillus subtilis and Lactococcus lactis of the DNase (streptodornase)-encoding gene from Streptococcus equisimilis H46A

AU - Wolinowska, Renata

AU - Cegłowski, Piotr

AU - Kok, Jan

AU - Venema, Gerhardus

N1 - Relation: http://www.rug.nl/gbb/ date_submitted:2007 Rights: University of Groningen, Groningen Biomolecular Sciences and Biotechnology Institute

PY - 1991/9/30

Y1 - 1991/9/30

N2 - A partial library of BclI-generated chromosomal DNA fragments from Streptococcus equisimilis H64A (Lancefield Group C) was constructed in Escherichia coli. Clones displaying either streptokinase or deoxyribonuclease (streptodornase; SDC) activities were isolated. The gene (sdc) expressing the SDC activity was allocated on the 1.1-kb AccI DNA subfragment. Sequence analysis of this DNA fragment revealed the presence of one open reading frame, which could encode a protein of 36.8 kDa. The N-terminal portion of the deduced protein exhibited features characteristic of prokaryotic signal peptides. The sdc gene was expressed in E. coli, Bacillus subtilis and Lactococcus lactis. As observed for S. equisimilis, in the heterologous Gram+ hosts, at least part of the SDC protein was secreted into the medium.

AB - A partial library of BclI-generated chromosomal DNA fragments from Streptococcus equisimilis H64A (Lancefield Group C) was constructed in Escherichia coli. Clones displaying either streptokinase or deoxyribonuclease (streptodornase; SDC) activities were isolated. The gene (sdc) expressing the SDC activity was allocated on the 1.1-kb AccI DNA subfragment. Sequence analysis of this DNA fragment revealed the presence of one open reading frame, which could encode a protein of 36.8 kDa. The N-terminal portion of the deduced protein exhibited features characteristic of prokaryotic signal peptides. The sdc gene was expressed in E. coli, Bacillus subtilis and Lactococcus lactis. As observed for S. equisimilis, in the heterologous Gram+ hosts, at least part of the SDC protein was secreted into the medium.

KW - GROUP-C STREPTOCOCCUS

KW - DEOXYRIBONUCLEASE

KW - STREPTOKINASE

KW - RECOMBINANT DNA

KW - SIGNAL PEPTIDE

KW - SECRETION

KW - STREPTOKINASE GENE

KW - NUCLEOTIDE-SEQUENCE

KW - CLONING VECTORS

KW - PLASMID DNA

KW - SYSTEM

KW - CONSTRUCTION

KW - RESISTANCE

KW - CLEAVAGE

KW - PROTEINS

KW - NUCLEASE

U2 - 10.1016/0378-1119(91)90574-U

DO - 10.1016/0378-1119(91)90574-U

M3 - Comment/Letter to the editor

VL - 106

SP - 115

EP - 119

JO - Gene

JF - Gene

IS - 1

ER -

Isolation, sequence and expression in Escherichia coli, Bacillus subtilis and Lactococcus lactis of the DNase (streptodornase)-encoding gene from Streptococcus equisimilis H46A

Abstract

Keywords

Access to Document

Handle.net

Embargoed Document

Fingerprint

Cite this