Kinetic studies on the transesterification of sunflower oil with 1-butanol catalyzed by Rhizomucor miehei lipase in a biphasic aqueous-organic system

M. Ilmi, A. Hommes, J. G. M. Winkelman, C. Hidayat, H. J. Heeres*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

9 Citations (Scopus)

Abstract

The kinetics of sunflower oil transesterification with 1-butanol using a homogeneous lipase (Rhizomucor miehei) in an aqueous-organic biphasic system were studied in a stirred batch reactor set-up. An initial screening study was performed to optimize relevant process conditions (enzyme concentration, stirring speed, 1-butanol to oil ratio). A kinetic model was developed based on concentration time profiles. Important reaction parameters were the oil concentration in n-hexane, the enzyme concentration, the 1-butanol to oil molar ratio; the organic to an aqueous solvent volume ratio, the temperature, the pH setting using a phosphate buffer and the agitation rate. The experimental data were modelled using the Ping Pong Bi Bi mechanism with non-competitive inhibition by 1-butanol and a term for irreversible enzyme deactivation during reaction. Agreement between model and experiments was good (R-2 = 0.99, average error 4.2%). (C) 2016 Published by Elsevier B.V.

Original languageEnglish
Pages (from-to)113-121
Number of pages9
JournalBiochemical Engineering Journal
Volume114
DOIs
Publication statusPublished - 15-Oct-2016

Keywords

  • Biphasic system
  • Biocatalysis
  • Rhizomucor miehei lipase
  • Substrate inhibition
  • Sunflower oil transesterification
  • Modelling
  • ENZYMATIC BIODIESEL PRODUCTION
  • CANDIDA-ANTARCTICA LIPASE
  • VEGETABLE-OILS
  • OLEIC-ACID
  • PALM OIL
  • ESTERIFICATION
  • MECHANISM
  • METHANOLYSIS
  • SEPARATOR
  • ETHANOL

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