KINETICS OF PERITONEAL B-1A CELLS (CD5 B-CELLS) IN YOUNG-ADULT MICE

GJ DEENEN*, FGM KROESE

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

32 Citations (Scopus)

Abstract

In the mouse, conventional B cells are continuously generated from precursor cells located in the bone marrow (BM), whereas the small subset of B-1 cells (formerly called Ly-1 B cells) constitute a self-replenishing population of cells. Here we studied the kinetics of murine peritoneal B-1a cells (i.e. B-1 cells expressing CD5). The actual number of B-1a cells in the peritoneal cavity that are proliferating, as detected by metaphase arrest and S-phase index, was below detection level, indicating that these cells do not divide significantly at this anatomical location. To establish the life-span of B-1a cells we used long-term administration of 5-bromo-2'-deoxyuridine in combination with three-color immunocytology on cytospin preparations. The renewal rate of peritoneal B-1a cells was 1.3% per day representing a 50% renewal time of 38 days. Splenic B cells and popliteal lymph node B cells (predominantly conventional B cells) showed an almost identical renewal rate of 1.1 % per day. The data show that peripheral B cells from various lymphoid tissues and locations do not differ significantly in their renewal capacity, even though there are differences in their developmental origin.

Original languageEnglish
Pages (from-to)12-16
Number of pages5
JournalEuropean Journal of Immunology
Volume23
Issue number1
Publication statusPublished - Jan-1993

Keywords

  • B-1 CELLS
  • KINETICS
  • IMMUNOCYTOLOGY
  • BROMODEOXYURIDINE
  • PERIPHERAL LYMPHOID ORGANS
  • BONE-MARROW
  • LY-1 B
  • POPULATION-KINETICS
  • MURINE-B
  • LYMPHOCYTES
  • MOUSE
  • BROMODEOXYURIDINE
  • PROLIFERATION
  • EXPRESSION

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