Lack of NMDA receptor subunit exchange alters Purkinje cell dendritic morphology in cerebellar slice cultures

F Metzger*, [No Value] Pieri, ULM Eisel, Isabelle Pieri

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

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Abstract

Early postnatal developmental changes in N-methyl-D-aspartate (NMDA) receptor (NR) subunits regulate cerebellar granule cell maturation and potentially Purkinje cell development. We therefore investigated Purkinje cell morphology in slice cultures from mice with genetic subunit exchange from NR2C to NP2B (NRX-2B). NR2C-2B Purkinje cells after 12 days in vitro showed a significantly impaired dendritic arbour complexity with reduced branching density as compared to wild-type cells, a phenotype that was reversed by NMDA treatment. These data support the concept that in cerebellar slice cultures, Purkinje cell dendritic outgrowth is regulated by granule cell inputs. (c) 2005 Elsevier B.V. All rights reserved.

Original languageEnglish
Pages (from-to)165-168
Number of pages4
JournalDevelopmental Brain Research
Volume155
Issue number2
DOIs
Publication statusPublished - 31-Mar-2005

Keywords

  • NMDA-NR2 subunits
  • organotypic slice cultures
  • dendritic differentiation
  • cerebellar granule cells
  • Purkinje cells
  • PROTEIN-KINASE-C
  • IN-VITRO
  • GROWTH
  • BRAIN
  • DIFFERENTIATION
  • EXPRESSION
  • MICE

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