SCOPE: Lactic acid bacteria (LAB) are recognized to promote gastrointestinal health by mechanisms that are not fully understood. LABs might modulate the mucus and thereby enhance intestinal barrier function. Herein, we investigated effects of different LAB strains and species on goblet cell genes involved in mucus synthesis.
METHODS AND RESULTS: Gene expression profiles of goblet cell-associated products (mucin MUC2, trefoil factor 3, resistin-like molecule β, carbohydrate sulfotransferase 5, and galactose-3-O-sulfotransferase 2) induced by LAB or their derived conditioned medium in human goblet cell line LS174T were studied. Effects of LAB on gene transcription were assessed with or without exposure to TNF-α, IL-13 or the mucus damaging agent tunicamycin. LAB did impact the related genes in a species and strain specific fashion and their effects were different in the presence of the cytokines and tunicamycin. Bioactive factors secreted by some strains were also found to regulate goblet cell-related genes.
CONCLUSION: Our findings provide novel insights in differences in modulatory efficacy on mucus genes between LAB species and strains. This study further unravels direct interactions between LAB and intestinal goblet cells, and highlights the importance of rationally selecting appropriate LAB candidates to achieve specific benefits in the gut. This article is protected by copyright. All rights reserved.
- Journal Article
- RESISTIN-LIKE MOLECULE
- PROTECTIVE FUNCTION
- ER STRESS