Lactobacilli: Important in biofilm formation on voice prostheses

Kevin J. D. A. Buijssen*, Hermie J. M. Harmsen, Henny C. van der Mei, Henk J. Busscher, Bernard F. A. M. van der Laan

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

15 Citations (Scopus)

Abstract

OBJECTIVE: We sought to identify bacterial strains responsible for biofilm formation on silicone rubber voice prostheses.

STUDY DESIGN: We conducted an analysis of the bacterial population in biofilms on used silicone rubber voice prostheses by using new microbiological methods.

METHODS: Two microbiological methods were used: polymerase chain reaction-denaturing gradient gel electrophoresis and fluorescence in situ hybridization. Twenty-six Provox2 and eight Groningen Ultra Low Resistance voice prostheses that were removed because of leakage through the prosthesis or because of increased airflow resistance were used in this study.

RESULTS: The results showed that 33 of the 34 explanted voice prosthetic biofilms contained lactobacilli in close association with the Candida sp. present.

CONCLUSION: Lactobacilli are general colonizers of tracheoesophaueal voice prostheses in vivo, growing intertwined with Candida. This knowledge may be important in the development of new pathways directed to prevent or to influence biofilm formation on tracheoesophageal voice prostheses and elongate their lifespan. (c) 2007 American Academy of Otolaryngology-Head and Neck Surgery Foundation. All rights reserved.

Original languageEnglish
Pages (from-to)505-507
Number of pages3
JournalOtolaryngology - Head and Neck Surgery
Volume137
Issue number3
DOIs
Publication statusPublished - Sep-2007

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