TY - JOUR
T1 - Lactobacillus diolivorans sp nov., a 1,2-propanediol-degrading bacterium isolated from aerobically stable maize silage
AU - Krooneman, J
AU - Faber, F
AU - Alderkamp, AC
AU - Elferink, SJHWO
AU - Driehuis, F
AU - Cleenwerck, [No Value]
AU - Swings, J
AU - Gottschal, JC
AU - Vancanneyt, M
PY - 2002/3
Y1 - 2002/3
N2 - Inoculation of maize silage with Lactobacillus buchneri (5 x 10(5) c.f.u. g(-1) of maize silage) prior to ensiling results in the formation of aerobically stable silage. After 9 months, lactic acid bacterium counts are approximately 10(10) c.f.u. g(-1) in these treated silages. An important subpopulation (5.9 x 10(7) c.f.u. g(-1)) is able to degrade 1,2-propanediol, a fermentation product of L. buchneri, under anoxic conditions to 1-propanol and propionic acid. From this group of 1,2-propanediol-fermenting, facultatively anaerobic, heterofermentative lactobacilli, two rod-shaped isolates were purified and characterized. Comparative 16S rDNA sequence analysis revealed that the newly isolated bacteria have identical 16S rDNA sequences and belong phylogenetically to the L. buchneri group. DNA-DNA hybridizations, whole-cell protein fingerprinting and examination of phenotypic properties indicated that these two isolates represent a novel species, for which the name Lactobacillus diolivorans sp. nov. is proposed. The type strain is LMG 19667(T) (= DSM 14421(T)).
AB - Inoculation of maize silage with Lactobacillus buchneri (5 x 10(5) c.f.u. g(-1) of maize silage) prior to ensiling results in the formation of aerobically stable silage. After 9 months, lactic acid bacterium counts are approximately 10(10) c.f.u. g(-1) in these treated silages. An important subpopulation (5.9 x 10(7) c.f.u. g(-1)) is able to degrade 1,2-propanediol, a fermentation product of L. buchneri, under anoxic conditions to 1-propanol and propionic acid. From this group of 1,2-propanediol-fermenting, facultatively anaerobic, heterofermentative lactobacilli, two rod-shaped isolates were purified and characterized. Comparative 16S rDNA sequence analysis revealed that the newly isolated bacteria have identical 16S rDNA sequences and belong phylogenetically to the L. buchneri group. DNA-DNA hybridizations, whole-cell protein fingerprinting and examination of phenotypic properties indicated that these two isolates represent a novel species, for which the name Lactobacillus diolivorans sp. nov. is proposed. The type strain is LMG 19667(T) (= DSM 14421(T)).
KW - lactic acid bacteria
KW - 1,2-propanediol degradation
KW - 1-propanol
KW - 16S rDNA
KW - identification
KW - DEOXYRIBONUCLEIC-ACID
KW - GENOMIC DNA
KW - HYBRIDIZATION
KW - FERMENTATION
KW - DEGRADATION
KW - BUCHNERI
KW - GROWTH
U2 - 10.1099/ijs.0.01979-0
DO - 10.1099/ijs.0.01979-0
M3 - Article
VL - 52
SP - 639
EP - 646
JO - International Journal of Systematic and Evolutionary Microbiology
JF - International Journal of Systematic and Evolutionary Microbiology
SN - 1466-5034
ER -