Lactobacillus diolivorans sp nov., a 1,2-propanediol-degrading bacterium isolated from aerobically stable maize silage

J Krooneman*, F Faber, AC Alderkamp, SJHWO Elferink, F Driehuis, [No Value] Cleenwerck, J Swings, JC Gottschal, M Vancanneyt

*Corresponding author for this work

    Research output: Contribution to journalArticleAcademicpeer-review

    125 Citations (Scopus)

    Abstract

    Inoculation of maize silage with Lactobacillus buchneri (5 x 10(5) c.f.u. g(-1) of maize silage) prior to ensiling results in the formation of aerobically stable silage. After 9 months, lactic acid bacterium counts are approximately 10(10) c.f.u. g(-1) in these treated silages. An important subpopulation (5.9 x 10(7) c.f.u. g(-1)) is able to degrade 1,2-propanediol, a fermentation product of L. buchneri, under anoxic conditions to 1-propanol and propionic acid. From this group of 1,2-propanediol-fermenting, facultatively anaerobic, heterofermentative lactobacilli, two rod-shaped isolates were purified and characterized. Comparative 16S rDNA sequence analysis revealed that the newly isolated bacteria have identical 16S rDNA sequences and belong phylogenetically to the L. buchneri group. DNA-DNA hybridizations, whole-cell protein fingerprinting and examination of phenotypic properties indicated that these two isolates represent a novel species, for which the name Lactobacillus diolivorans sp. nov. is proposed. The type strain is LMG 19667(T) (= DSM 14421(T)).

    Original languageEnglish
    Pages (from-to)639-646
    Number of pages8
    JournalInternational Journal of Systematic and Evolutionary Microbiology
    Volume52
    DOIs
    Publication statusPublished - Mar-2002

    Keywords

    • lactic acid bacteria
    • 1,2-propanediol degradation
    • 1-propanol
    • 16S rDNA
    • identification
    • DEOXYRIBONUCLEIC-ACID
    • GENOMIC DNA
    • HYBRIDIZATION
    • FERMENTATION
    • DEGRADATION
    • BUCHNERI
    • GROWTH

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