Let op! Cell wall under construction: Untangling Bacillus subtilis cell wall synthesis

Danae Morales Angeles

Research output: ThesisThesis fully internal (DIV)

500 Downloads (Pure)

Abstract

The shape of the bacterial cell is determined by a cell wall composed of peptidoglycan (PG). PG synthesis involves the synthesis of its building block (Lipid II) in the cytoplasm, which is flipped over the membrane and incorporated into the cell wall by the Penicillin Binding Proteins (PBPs). The study of cell wall synthesis helps to understand the increased bacterial resistance to current antibiotics, and to develop new antibiotics. In this thesis, three lines of research are worked out, using the Gram-positive rod-shaped organism Bacillus subtilis as a model.
The first research line explored the mode of action of nisin, a lantibiotic with a pore-forming capacity, which also clusters Lipid II in the membrane. Our data showed that both processes are linked. The second line studied chemical differences in cell wall composition. We showed that the composition of the division septum differs from the lateral wall, as it is enriched in material that is not yet processed or crosslinked. Finally, the function of the PBP2B PASTA domains was analysed. We showed that although the PASTA domains are not required for PBP2B localization, they do play a role in cell division, most likely by interacting with the cell division protein DivIB.
Original languageEnglish
QualificationDoctor of Philosophy
Awarding Institution
  • University of Groningen
Supervisors/Advisors
  • Scheffers, Dirk-Jan, Supervisor
  • Driessen, Arnold, Supervisor
Award date12-Oct-2018
Place of Publication[Groningen]
Publisher
Print ISBNs9789403409337
Electronic ISBNs9789403409320
Publication statusPublished - 2018

Cite this