Light and electron microscopical demonstration of the ouabain-sensitive, potassium-dependent p-nitrophenylphosphatase activity (K-NPPase) using a Ce-Mg-double capture technique

M GLIESING*, D. Kalicharan, KJ HALBHUBER

*Corresponding author for this work

    Research output: Contribution to journalComment/Letter to the editorAcademicpeer-review

    Abstract

    The cerium-based method of Kobayashi et al. for the histochemical demonstration of K-NPPase activity was improved. Besides Ce3+ additionally Mg2+ ions as orthophosphate capture were employed (double capture technique). For light microscopical purposes the Mg-phosphate was converted into Ce-phosphate by treatment of the sections with Ce-citrate yielding higher quantity of reaction product. Unspecific background staining was eliminated by EGTA. In the electron microscope this technique brought about fine granular reaction products without diffusion artefacts.

    Original languageEnglish
    Pages (from-to)867-874
    Number of pages8
    JournalCellular and molecular biology
    Volume41
    Issue number6
    Publication statusPublished - Sep-1995

    Keywords

    • NA-K-ATPASE
    • K+-DEPENDENT PHOSPHATASE
    • P-NITROPHENYLPHOSPHATASE
    • CERIUM
    • RAT KIDNEY
    • PHOSPHATASE HISTOCHEMISTRY
    • CYTOCHEMICAL LOCALIZATION
    • ADENOSINE-TRIPHOSPHATASE

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