Light-microscopic and electron-microscopic evaluation of short-term nerve regeneration using a biodegradable poly(DL-lactide-epsilon-caprolacton) nerve guide

W. F. A. den Dunnen, I. Stokroos, E. H. Blaauw, A. Holwerda, A. J. Pennings, P. H. Robinson, J. M. Schakenraad

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    The aim of this study was to evaluate short-term peripheral nerve regeneration across a IO-mm gap, using a biodegradable poly(DL-lactide-epsilon-caprolacton) nerve guide, with an internal diameter of 1.5 mm and a wall thickness of 0.30 mm. To do so, we evaluated regenerating nerves using light microscopy, transmission electron microscopy and morphemetric analysis after implantation of 12-mm nerve guides in the sciatic nerve of the rat. Evaluation times ranged from 3-10 weeks. Three weeks after reconstruction, myelinated nerve fibers could be observed in the distal nerve stump. Ten weeks after reconstruction, the regenerating nerves already resembled normal nerves. In conclusion, we show that poly(DL-lactide-epsilon-caprolacton) nerve guides can be successfully applied in the reconstruction of severed nerves in the rat model. Furthermore, we have observed the fastest nerve regeneration described thus far, after reconstruction using a biodegradable nerve guide. (C) 1996 John Wiley & Sons, Inc.

    Original languageEnglish
    Pages (from-to)105-115
    Number of pages11
    JournalJournal of Biomedical Materials Research
    Issue number1
    Publication statusPublished - May-1996


    • GROWTH
    • MODEL
    • RAT

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