Limitations of the nested reverse transcriptase polymerase chain reaction on tyrosinase for the detection of malignant melanoma micrometastases in lymph nodes

A Calogero, H Timmer-Bosscha, ATMG Tiebosch, NH Mulder, GAP Hospers*, H. Schraffordt Koops

*Corresponding author for this work

    Research output: Contribution to journalArticleAcademicpeer-review

    17 Citations (Scopus)
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    Abstract

    The specificity and sensitivity of the nested reverse transcriptase polymerase chain reaction (RT-PCR) on tyrosinase was studied, for the detection of micrometastases of malignant melanoma. The specificity was assessed in the blood of six healthy donors, four patients with non-melanoma cancers of which one patient was treated with granulocyte-colony stimulating factor. Lymph nodes of nine patients without malignant melanoma were tested and four cell lines of various other tumours. Six of the nine non-melanoma lymph nodes were positive in this assay. The sensitivity was tested in a spike experiment in vitro, using a melanoma cell line. The detection limit was ten melanoma cells per 10(7) peripheral blood lymphocytes. (C) 2000 Cancer Research Campaign.

    Original languageEnglish
    Pages (from-to)184-187
    Number of pages4
    JournalBritish Jounal of Cancer
    Volume83
    Issue number2
    DOIs
    Publication statusPublished - Jul-2000

    Keywords

    • tyrosinase
    • melanoma
    • lymph nodes
    • nested RT-PCR
    • PERIPHERAL-BLOOD
    • MESSENGER-RNA
    • RT-PCR
    • TUMOR PROGRESSION
    • SENTINEL NODES
    • CLINICAL STAGE
    • CELL-LINES
    • MARKER
    • IMMUNOHISTOCHEMISTRY
    • MART-1

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