Abstract
The specificity and sensitivity of the nested reverse transcriptase polymerase chain reaction (RT-PCR) on tyrosinase was studied, for the detection of micrometastases of malignant melanoma. The specificity was assessed in the blood of six healthy donors, four patients with non-melanoma cancers of which one patient was treated with granulocyte-colony stimulating factor. Lymph nodes of nine patients without malignant melanoma were tested and four cell lines of various other tumours. Six of the nine non-melanoma lymph nodes were positive in this assay. The sensitivity was tested in a spike experiment in vitro, using a melanoma cell line. The detection limit was ten melanoma cells per 10(7) peripheral blood lymphocytes. (C) 2000 Cancer Research Campaign.
Original language | English |
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Pages (from-to) | 184-187 |
Number of pages | 4 |
Journal | British Jounal of Cancer |
Volume | 83 |
Issue number | 2 |
DOIs | |
Publication status | Published - Jul-2000 |
Keywords
- tyrosinase
- melanoma
- lymph nodes
- nested RT-PCR
- PERIPHERAL-BLOOD
- MESSENGER-RNA
- RT-PCR
- TUMOR PROGRESSION
- SENTINEL NODES
- CLINICAL STAGE
- CELL-LINES
- MARKER
- IMMUNOHISTOCHEMISTRY
- MART-1