Lipid Fingerprints and Cofactor Dynamics of Light-Harvesting Complex II in Different Membranes

Sebastian Thallmair*, Petteri A. Vainikka, Siewert J. Marrink

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

10 Citations (Scopus)
49 Downloads (Pure)

Abstract

Plant light-harvesting complex II (LHCII) is the key antenna complex for plant photosynthesis. We present coarse-grained molecular dynamics simulations of monomeric and trimeric LHCII in a realistic thylakoid membrane environment based on the Martini force field. The coarse-grained protein model has been optimized with respect to atomistic reference simulations. Our simulations provide detailed insights in the thylakoid lipid fingerprint of LHCII which compares well with experimental data from membrane protein purification. Comparing the monomer and trimeric LHCII reveals a stabilizing effect of trimerization on the chromophores as well as the protein. Moreover, the average chromophore distance shortens in the trimer leading to stronger excitonic couplings. When changing the native thylakoid environment to a model membrane the protein flexibility remains constant, whereas the chromophore flexibility is reduced. Overall, the presented LHCII model lays the foundation to investigate the μs dynamics of this key antenna protein of plants.

Original languageEnglish
Article numberj.bpj.2019.03.009
Pages (from-to)1446-1455
Number of pages10
JournalBiophysical Journal
Volume116
Issue number8
Early online date22-Mar-2019
DOIs
Publication statusPublished - Apr-2019

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