LIVER METASTASIS MODEL OF COLON-CANCER IN THE RAT - IMMUNOHISTOCHEMICAL CHARACTERIZATION

C THOMAS*, AM NIJENHUIS, W TIMENS, PJK KUPPEN, T DAEMEN, GL SCHERPHOF

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

49 Citations (Scopus)

Abstract

Inoculation of 3 x 10(4) to 3 x 10(5) CC531 colon adenocarcinoma cells into the portal vein of syngeneic WAG/Rij rats provides a reproducible animal model of colon cancer liver metastasis with macroscopically visible tumor nodules at day 25 after inoculation. In this study, the inflammatory cell response immune reactive cells accumulated in the liver sinusoids of tumor-bearing livers, especially resident Kupffer cells (KC; ED1+/ED2+), and to a lesser extent pit cells (OX8+/OX19-), T lymphocytes (OX8+lOX19+) and polymorphonuclear leukocytes (PMN; HIS48+). All these cell types may be involved in the lysis of CC531 cells in the liver, although only major histocompatibility complex class II+ monocytes (3D11+/ED1+/ED2-), but not resident KC, T lymphocytes nor PMN, accumulated at the peritumoral area between liver parenchyma and tumor foci. Especially monocytes but also PMN, lymphocytes and pit cells appeared to infiltrate the stromal tumor compartment. In view of the low cell dose needed, the rat liver metastasis model of colon cancer used in this study reflects well the human, weakly immunogenic, tumor-host relationships and provides an excellent opportunity for the study of cellular reactions related to chemo- and/or immunotherapy protocols.

Original languageEnglish
Pages (from-to)102-112
Number of pages11
JournalInvasion & metastasis
Volume13
Issue number2
Publication statusPublished - 1993

Keywords

  • EXPERIMENTAL LIVER METASTASIS
  • CC531 COLON ADENOCARCINOMA
  • IMMUNOHISTOCHEMISTRY
  • WAG/RIJ RATS
  • KUPFFER CELLS
  • MONOCYTES
  • LYMPHOCYTES
  • PIT CELLS
  • POLYMORPHONUCLEAR LEUKOCYTES
  • NATURAL-KILLER CELLS
  • MONOCLONAL-ANTIBODY
  • TUMOR SITE
  • MACROPHAGES
  • SUBPOPULATIONS
  • RESECTION
  • CARCINOMA
  • CYTOKINES
  • INVITRO

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