LOCALIZATION OF THE PATHWAY OF THE PENICILLIN BIOSYNTHESIS IN PENICILLIUM-CHRYSOGENUM

WH MULLER*, TP VANDERKRIFT, AJJ KROUWER, HAB WOSTEN, LHM VANDERVOORT, EB SMAAL, AJ VERKLEIJ

*Corresponding author for this work

    Research output: Contribution to journalArticleAcademicpeer-review

    130 Citations (Scopus)

    Abstract

    The localization of the enzymes involved in penicillin biosynthesis in Penicillium chrysogenum hyphae has been studied by immunological detection methods in combination with electron microscopy and cell fractionation. The results suggest a complicated pathway involving different intracellular locations. The enzyme-delta-(L-alpha-aminoadipyl)-L-cysteinyl-D-valine synthetase was found to be associated with membranes or small organelles. The next enzyme isopenicillin N-synthetase appeared to be a cytosolic enzyme. The enzyme which is involved in the last step of penicillin biosynthesis, acyltransferase, was located in organelles with a diameter of 200-800 nm. These organelles, most probably, are microbodies. A positive correlation was found between the capacity for penicillin production and the number of organelles per cell when comparing different P. chrysogenum strains.

    Original languageEnglish
    Pages (from-to)489-495
    Number of pages7
    JournalEMBO Journal
    Volume10
    Issue number2
    Publication statusPublished - Feb-1991

    Keywords

    • COMPARTMENTALIZATION
    • MICROBODIES
    • PENICILLIN BIOSYNTHESIS
    • ISOPENICILLIN-N SYNTHETASE
    • ENZYME
    • DELTA-(L-ALPHA-AMINOADIPYL)-L-CYSTEINYL-D-VALINE
    • GENE
    • EFFICIENCY
    • CLONING
    • YEAST
    • ACID

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