Low cost sequencing of mitogenomes from museum samples using baits capture and Ion Torrent

Spyros Kollias, Marloes Poortvliet, Irina Smolina, Galice Hoarau*

*Corresponding author for this work

    Research output: Contribution to journalArticleAcademicpeer-review

    5 Citations (Scopus)
    273 Downloads (Pure)

    Abstract

    The development of various target enrichment methods in combination with next generation sequencing techniques has greatly facilitated the use of partially degraded DNA samples in genetic studies. We employed the MYbaits target enrichment system in combination with Ion Torrent sequencing on a broad range of DNA quality, extracted from tissues obtained from both natural history archives and through various opportunistic sampling methods, to sequence the mitogenome of 11 mobulid rays and two closely related species. Mobulids are large, elusive pelagic filter feeders, for which conservation concerns have recently be raised in connection to their vulnerable life histories and increasing fishing pressure. We show that the MYbaits target enrichment method can be used to effectively sequence large parts of the mitogenome from heavily degraded DNA samples, and provide a time and cost effective alternative for genetic studies of rare and/or difficult to sample species.

    Original languageEnglish
    Pages (from-to)345-348
    Number of pages4
    JournalConservation genetics resources
    Volume7
    Issue number2
    DOIs
    Publication statusPublished - Jun-2015

    Keywords

    • Next generation sequencing
    • Targeted sequencing
    • MYbaits
    • Mitogenome
    • Manta
    • Mobula
    • SPECIMENS

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