TY - JOUR
T1 - Low-load compression testing
T2 - A novel way of measuring Biofilm thickness
AU - Paramonova, Ekaterina
AU - De Jong, Ed D.
AU - Krom, Bastiaan P.
AU - Van der Mei, Henny C.
AU - Busscher, Henk J.
AU - Sharma, Prashant K.
PY - 2007/11
Y1 - 2007/11
N2 - Biofilms are complex and dynamic communities of microorganisms that are studied in many fields due to their abundance and economic impact. Biofilm thickness is an important parameter in biofilm characterization. Current methods of measuring biofilm thicknesses have several limitations, including application, availability, and costs. Here, we present low-load compression testing (LLCT) as a new method for measuring biofilm thickness. With LLCT, biofilm thicknesses are measured during compression by inducing small loads, up to 5 Pa, corresponding to 0.1% deformation, making LLCT essentially a nondestructive technique. Comparison of the thicknesses of various bacterial and yeasts biofilms obtained by LLCT and by using confocal laser scanning microscopy (CLSM) resulted in the conclusion that CLSM underestimates the biofilm thickness due to poor penetration of different fluorescent dyes, especially through the thicker biofilms, whereas LLCT does not suffer from this thickness limitation.
AB - Biofilms are complex and dynamic communities of microorganisms that are studied in many fields due to their abundance and economic impact. Biofilm thickness is an important parameter in biofilm characterization. Current methods of measuring biofilm thicknesses have several limitations, including application, availability, and costs. Here, we present low-load compression testing (LLCT) as a new method for measuring biofilm thickness. With LLCT, biofilm thicknesses are measured during compression by inducing small loads, up to 5 Pa, corresponding to 0.1% deformation, making LLCT essentially a nondestructive technique. Comparison of the thicknesses of various bacterial and yeasts biofilms obtained by LLCT and by using confocal laser scanning microscopy (CLSM) resulted in the conclusion that CLSM underestimates the biofilm thickness due to poor penetration of different fluorescent dyes, especially through the thicker biofilms, whereas LLCT does not suffer from this thickness limitation.
KW - MICROBIAL BIOFILMS
KW - QUANTIFICATION
KW - MICROSCOPY
U2 - 10.1128/AEM.00935-07
DO - 10.1128/AEM.00935-07
M3 - Article
SN - 0099-2240
VL - 73
SP - 7023
EP - 7028
JO - Applied and environmental microbiology
JF - Applied and environmental microbiology
IS - 21
ER -