Low sensitivity of type VII collagen enzyme-linked immunosorbent assay in epidermolysis bullosa acquisita: serration pattern analysis on skin biopsy is required for diagnosis

J. B. Terra*, M. F. Jonkman, G. F. H. Diercks, H. H. Pas

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

30 Citations (Scopus)

Abstract

BackgroundThe type VII collagen (coll VII) enzyme-linked immunosorbent assay (ELISA) has been reported to have high sensitivity (>93%) and specificity (>96%) for diagnosing epidermolysis bullosa acquisita (EBA) in patients who are seropositive on indirect immunofluorescence on salt-split skin (SSS).

ObjectivesTo investigate the added value of the coll VII ELISA in the laboratory diagnosis of SSS-positive and SSS-negative EBA and to correlate the ELISA index with disease episode.

MethodsThe coll VII ELISA was performed on banked sera of 28 patients with EBA: 15 SSS positive and 13 SSS negative. Sera from healthy blood donors (n=17) and patients with other autoimmune blistering diseases (n=29) served as controls. In four patients, the ELISA index was measured longitudinally. Serration pattern analysis by direct immunofluorescence has been prospectively performed since 2000 in 19 patients.

ResultsThe sensitivity in the SSS-positive group was 80% whereas it was 23% in the SSS-negative group. In the prospective EBA subset it was 45%. The sensitivity of u-serration pattern analysis on skin biopsy was 89%. Ten (53%) of these cases were seronegative with both ELISA and SSS, and would have been missed by serum analysis alone. Of the 46 control sera, one serum tested positive (specificity 978%). The coll VII ELISA correlated with disease activity over time in individual patients.

ConclusionsThe coll VII ELISA has limited added value in SSS-negative EBA cases. The ELISA test is valuable in differentiating EBA from antilaminin-332 mucous membrane pemphigoid and anti-p200 pemphigoid and in its ability to monitor patients with EBA serologically. U-serration pattern analysis on immunofluorescence skin biopsy is the gold standard for the diagnosis of EBA.

Original languageEnglish
Pages (from-to)164-167
Number of pages4
JournalBRITISH JOURNAL OF DERMATOLOGY
Volume169
Issue number1
DOIs
Publication statusPublished - Jul-2013

Keywords

  • DIRECT IMMUNOFLUORESCENCE
  • NC2 DOMAINS
  • ELISA
  • AUTOANTIBODIES
  • MICROSCOPY
  • ANTIBODIES
  • SEVERITY
  • DISEASE

Cite this